a Universite Grenoble Alpes - LBFA and BEeSy, PROMETHEE, Proteomic Platform , Saint-Martin-d'Heres , France.
b Inserm, U1055, PROMETHEE Proteomic Platform , Saint-Martin-d'Heres , France.
Expert Rev Proteomics. 2019 Feb;16(2):139-159. doi: 10.1080/14789450.2019.1559061. Epub 2018 Dec 24.
Acetylation is a widely occurring post-translational modification (PTM) of proteins that plays a crucial role in many cellular physiological and pathological processes. Over the last decade, acetylation analyses required the development of multiple methods to target individual acetylated proteins, as well as to cover a broader description of acetylated proteins that comprise the acetylome. Areas covered: This review discusses the different types of acetylation (N-ter/K-/O-acetylation) and then describes some major strategies that have been reported in the literature to detect, enrich, identify and quantify protein acetylation. The review highlights the advantages and limitations of these strategies, to guide researchers in designing their experimental investigations and analysis of protein acetylation. Finally, this review highlights the main applications of acetylomics (proteomics based on mass spectrometry) for understanding physiological and pathological conditions. Expert opinion: Recent advances in acetylomics have enhanced knowledge of the biological and pathological roles of protein acetylation and the acetylome. Besides, radiolabeling and western blotting remain also techniques-of-choice for targeted protein acetylation. Future challenges in acetylomics to analyze the N-ter and K-acetylome will most likely require enrichment/fractionation, MS instrumentation and bioinformatics. Challenges also remain to identify the potential biological roles of O-acetylation and cross-talk with other PTMs.
乙酰化是一种广泛存在的蛋白质翻译后修饰（PTM），在许多细胞生理和病理过程中起着至关重要的作用。在过去的十年中，乙酰化分析需要开发多种方法来靶向单个乙酰化蛋白质，以及更广泛地描述构成乙酰组的乙酰化蛋白质。涵盖领域：本文综述了不同类型的乙酰化（N 端/K 端/O 端乙酰化），然后描述了文献中报道的一些主要策略，用于检测、富集、鉴定和定量蛋白质乙酰化。该综述强调了这些策略的优缺点，以指导研究人员设计他们的实验研究和蛋白质乙酰化分析。最后，本文综述了乙酰组学（基于质谱的蛋白质组学）在理解生理和病理条件中的主要应用。专家意见：乙酰组学的最新进展增强了对蛋白质乙酰化和乙酰组生物学和病理学作用的认识。此外，放射性标记和蛋白质印迹仍然是靶向蛋白质乙酰化的首选技术。未来分析 N 端和 K 端乙酰组学的挑战可能需要富集/分级、MS 仪器和生物信息学。鉴定 O 乙酰化的潜在生物学作用以及与其他 PTM 的相互作用仍然是挑战。
