Al-Azzawi Mahmood A, Ghoneim Adel H, Elmadbouh Ibrahim
Department of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, Sadat, Egypt.
Departments of Medical Biochemistry, College of Medicine, Al-Mustansiriyah University, Baghdad, Iraq.
J Med Biochem. 2017 Oct 28;36(4):331-340. doi: 10.1515/jomb-2017-0012. eCollection 2017 Oct.
BACKGROUND: The aim was to evaluate the association of plasma 25-hydroxyvitamin D (25-OHD) and vitamin D binding protein (VDBP or Gc-globin) gene polymorphism with oxidant-antioxidant profiles in patients with chronic obstructive pulmonary disease (COPD), and their role as biomarker risk factors in susceptibility and severity of COPD. METHODS: Eighty patients diagnosed with COPD (mild, moderate and severe according to lung function tests; FEV 1%) and 80 healthy controls were included in the study. Serum nitric oxide (NO) and lipid peroxide (LP), plasma reduced glutathione (RGSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activity, 25-OHD and VDBP polymorphism were analyzed in all subjects. RESULTS: COPD patients had significantly decreased serum NO, plasma SOD, RGSH, GSH-Px, CAT and 25-OHD versus controls, but had significantly increased serum LP. In COPD patients, 25-OHD levels were significantly lower (41.49± 13.65 ng/mL) versus controls, but more lower in severe COPD patients (30.54±9.09 ng/mL; sensitivity 79.2%; spe - cificity 73.2%, p<0.001) versus mild and moderate COPD. VDBP genotypes frequencies were Gc1S-1S=23.8%, Gc1F-1S=28.8%, Gc1F-1F=15%, Gc1S-2=20%, Gc1F-2=11.3% and Gc2-2=1.3%. Also, VDBP variants frequencies were Gc1S=48.1%, Gc1F=35% and Gc2=16.6%. How ever, Gc1F-1S genotypes and Gc1F variants were significantly higher than in controls (10%, 12.5%; p=0.009, p=0.001, respectively). Moreover, in severe COPD patients, Gc1F-1S genotype was significantly higher than in mild COPD (41.7% vs 31.3%, p=0.04). CONCLUSION: COPD patients had significantly lower plasma 25-OHD and were associated with significantly higher VDBP Gc1F-1S genotypes and Gc1F variants frequencies than controls. Low vitamin D levels and VDBP polymorphism may be important as diagnostic risk factors in the susceptibility to and severity of COPD.
背景：目的是评估慢性阻塞性肺疾病（COPD）患者血浆25-羟基维生素D（25-OHD）和维生素D结合蛋白（VDBP或Gc球蛋白）基因多态性与氧化-抗氧化谱的关联，以及它们作为生物标志物风险因素在COPD易感性和严重程度中的作用。 方法：本研究纳入80例经诊断为COPD的患者（根据肺功能测试分为轻度、中度和重度；FEV 1%）和80名健康对照者。分析了所有受试者的血清一氧化氮（NO）和脂质过氧化物（LP）、血浆还原型谷胱甘肽（RGSH）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性、25-OHD和VDBP多态性。 结果：与对照组相比，COPD患者的血清NO、血浆SOD、RGSH、GSH-Px、CAT和25-OHD显著降低，但血清LP显著升高。在COPD患者中，25-OHD水平显著低于对照组（41.49±13.65 ng/mL），但重度COPD患者更低（30.54±9.09 ng/mL；敏感性79.2%；特异性73.2%，p<0.001），与轻度和中度COPD患者相比。VDBP基因型频率为Gc1S-1S=23.8%，Gc1F-1S=28.8%，Gc1F-1F=15%，Gc1S-2=20%，Gc1F-2=11.3%，Gc2-2=1.3%。此外，VDBP变异频率为Gc1S=48.1%，Gc1F=35%，Gc2=16.6%。然而，Gc1F-1S基因型和Gc1F变异显著高于对照组（分别为10%，12.5%；p=0.009，p=0.001）。此外，在重度COPD患者中，Gc1F-1S基因型显著高于轻度COPD患者（41.7%对31.3%，p=0.04）。 结论：与对照组相比，COPD患者的血浆25-OHD显著降低，且VDBP Gc1F-1S基因型和Gc1F变异频率显著更高。低维生素D水平和VDBP多态性可能作为COPD易感性和严重程度的诊断风险因素具有重要意义。
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