Stepanova Ye I, Kolpakov I Ye, Zyhalo V M, Vdovenko V Yu, Vasylenko V V, Leonovych O M
State Institution «National Research Center for Radiation Medicine of the National Academy of Medical Sciences of Ukraine», 53 Melnykova str., Kyiv, 04050, Ukraine.
Probl Radiac Med Radiobiol. 2018 Dec;23:462-470. doi: 10.33145/2304-8336-2018-23-462-470.
To determine the features of the nitrogen oxide metabolism and risk of developing endothelial dysfunc-tion in children with e-NOS 4a/4b gene polymorphism, who live under prolonged enter 137Cs to the body.
There were examined 117 children-residents of radioactively contaminated territories and50 children of control group. The level of stable metabolites was defined in blood serum (NO2- and NO3-). The ther-mographic method was used to register the endothelium dependent reaction of the vascular bed to changes in theblood supply. The ventilation capacity of the lungs was evaluated using this method of pneumotachography.Polymorphism in intron 4 of the gene e-NOS was studied by the method of polymerase chain reaction. The contentof 137Cs in the body of children was determined using a human radiation counter Skrynner M-3.
In children-residents of radioactively contaminated territories with genotype 4a/4b com-paring to children who had genotype 4b/4b, the decrease in the nitric content of in the blood serum, the increase inthe thermographic index of the recovery period of blood circulation to the baseline level after occlusion test werenoted, that is indicative of the decreased NO-synthase active of vascular endothelium in the carriers of the minorallele a in the 4th intron of gene eNOS (genotype 4a/4b), and is a risk factor for development of endothelial dysfunc-tion. It was proved a decrease in the index of lung tissue elasticity and stretchability - FVC / NFVC of the lungs com-paring to children with genotype 4b/4b, there was a reduction of integral index of respiratory tract permeability -FEV1/NFEV1. The inverse correlation dependence between the presence of allele a in the genotype and the values ofFVC/NFVC of the lungs (r = -0.259; p <0.05) and FEV1/NFEV1 (r = -0.2267; p <0.05) was found. Signs of bron-chospasm were found in the carriers of the allele a in 1.5 times more often than in children-carriers of homozy-gotes from allele b.
确定长期受体内摄入137Cs影响且携带e-NOS 4a/4b基因多态性的儿童的氮氧化物代谢特征及发生内皮功能障碍的风险。
对117名来自放射性污染地区的儿童居民和50名对照组儿童进行了检查。测定血清中稳定代谢物（NO2-和NO3-）的水平。采用热成像法记录血管床对血液供应变化的内皮依赖性反应。使用呼吸流速描记法评估肺通气能力。通过聚合酶链反应法研究e-NOS基因第4内含子的多态性。使用Skrynner M-3人体辐射计数器测定儿童体内137Cs的含量。
与携带4b/4b基因型的儿童相比，携带4a/4b基因型的来自放射性污染地区的儿童居民血清中一氧化氮含量降低，闭塞试验后血液循环恢复到基线水平的热成像指数增加，这表明eNOS基因第4内含子中次要等位基因a的携带者血管内皮一氧化氮合酶活性降低，是发生内皮功能障碍的危险因素。与携带4b/4b基因型的儿童相比，已证实肺组织弹性和伸展性指数——肺用力肺活量/非用力肺活量降低，呼吸道通透性综合指数——第一秒用力呼气容积/非第一秒用力呼气容积降低。发现基因型中存在等位基因a与肺用力肺活量/非用力肺活量值（r = -0.259；p <0.05）和第一秒用力呼气容积/非第一秒用力呼气容积值（r = -0.2267；p <0.05）呈负相关。发现携带等位基因a的儿童出现支气管痉挛的迹象比携带等位基因b的纯合子儿童多1.5倍。
