Stepanova Ye I, Kolpakov I Ye, Zygalo V M, Vdovenko V Yu, Kondrashova V H, Leonovich O S, Kryzhanivska V V
State Institution «National Research Center for Radiation Medicine of National Academy of Medical.
Probl Radiac Med Radiobiol. 2017 Dec;22:463-475.
to determine the effectiveness of citrulline use for correcting endothelial dysfunction in children resi dents of radioactively contaminated areas.
A group of children residents of radioactively contaminated areas with the presence of clinical and paraclinical signs of endothelial dysfunction was selected to assess the effectiveness of correcting endothelial dysfunction by the usage of NO potential donator - citrulline according to the data of selective screen ing. There were determined the biochemical parameters of the content of NO stable metabolites, L arginine, lipid peroxidation, antioxidant enzymes in the blood serum; the indices of cellular and humoral immunity; the instrumen tal indices of vascular endothelium dependent reaction on occlusion test, the lung ventilation capacity, the bioelec tric activity of the myocardium, the autonomic regulation of the cardiovascular system.Examined children were received a course of citrulline malate.
An increased content of serum L arginine, nitrite, and amounts of NO metabolites was established in children with endothelial dysfunction who were received a course of citrulline malate. Bronchospasm elimination was noted in the significant part of examined patients after the drug use. Decreased recovery period and increased period of hypercompensation for thermographic circulation index in the test with post occlusion reac tive hyperemia were detected by an evaluation of indicators for vascular endothelium dependent vasodilatation using thermographic method indicating an increased endothelial vasomotor capacity. There was tendency to improve the processes of autonomic regulation of the heart rhythm and repolarization of the heart muscle. The antioxidant effect of used citrulline malate course was determined as: decreased content of serum LPO end products that react with thiobarbituric acid under elevated activity of antioxidant - catalase. An increase in the percentage of T lymphocyte, normalization of their subpopulation composition was noted in dynamics of citrulline malate application.
确定瓜氨酸对纠正受放射性污染地区儿童内皮功能障碍的有效性。
根据选择性筛查数据，选择一组有内皮功能障碍临床和准临床症状的受放射性污染地区儿童居民，以评估使用一氧化氮潜在供体——瓜氨酸纠正内皮功能障碍的有效性。测定血清中一氧化氮稳定代谢产物、L-精氨酸、脂质过氧化、抗氧化酶的生化参数；细胞和体液免疫指标；血管内皮依赖性反应在闭塞试验中的仪器指标、肺通气能力、心肌生物电活动、心血管系统自主调节。受试儿童接受了一个疗程的苹果酸瓜氨酸治疗。
接受一个疗程苹果酸瓜氨酸治疗的内皮功能障碍儿童血清L-精氨酸、亚硝酸盐含量及一氧化氮代谢产物量增加。用药后，大部分受试患者的支气管痉挛得到缓解。通过热成像法评估血管内皮依赖性血管舒张指标，发现在闭塞后反应性充血试验中，热成像循环指数的恢复期缩短，高代偿期延长，表明内皮血管舒缩能力增强。心律自主调节和心肌复极过程有改善趋势。所用苹果酸瓜氨酸疗程的抗氧化作用表现为：在抗氧化酶——过氧化氢酶活性升高的情况下，与硫代巴比妥酸反应的血清脂质过氧化终产物含量降低。在苹果酸瓜氨酸应用过程中，T淋巴细胞百分比增加，其亚群组成正常化。
