Jahagirdar Devashree, Gore Charusheela R, Patel Himadri, Maria Kunjal, Tandon Ishita, Sharma Nilesh K
Cancer and Translational Research Lab, Dr D.Y. Patil Biotechnology and Bioinformatics Institute, Dr D.Y. Patil Vidyapeeth, India. Email:
Asian Pac J Cancer Prev. 2018 Dec 25;19(12):3307-3316. doi: 10.31557/APJCP.2018.19.12.3307.
Background: There are evidences on the role of extracellular factors in cellular communication between cancer cells and non-cancerous cells to support tumor progression and a phenomenon of cancer cachexia. However, evidences are scarce to show the effects of extracellular factors from one carcinoma microenvironment upon growth and survival of another carcinoma. Methodology: To address the above issue, we have selected excised breast carcinoma tissue samples and in vitro grown MCF-7 sources of extracellular factors and tested their effects to evaluate growth and proliferation inhibitory potential against a cervical carcinoma cell line HeLa. Results: Data from the in vitro experiments like Trypan blue dye exclusion, MTT assay, cell cycle assay and annexin V/PI staining lead us to suggest that the extracellular factors collected from the culture medium of in vitro grown MCF-7 and excised breast carcinoma tissue play an apoptosis inducing and cell cycle arrest role in HeLa. In these in vitro experiments, we detected the presence of up to 40-50% apoptotic cell death in HeLa cells and increase in G2-M cell cycle phase from 11%-25% due to treatment with extracellular factors from human breast carcinoma cells. Discussion and Conclusion: These observations are novel and suggest that extracellular factors from breast carcinoma play an apoptosis inducing and growth inhibitory role upon on HeLa cells. This study can also support the concept of cancer cachexia and a possible hypothesis for rare chance of synchronous two or more primary tumor in a single patient.
有证据表明细胞外因子在癌细胞与非癌细胞之间的细胞通讯中发挥作用，以支持肿瘤进展和癌症恶病质现象。然而，关于一种癌微环境中的细胞外因子对另一种癌的生长和存活的影响的证据却很少。方法：为了解决上述问题，我们选择了切除的乳腺癌组织样本和体外培养的MCF-7细胞作为细胞外因子来源，并测试它们对宫颈癌细胞系HeLa的生长和增殖抑制潜力的影响。结果：台盼蓝拒染法、MTT法、细胞周期分析和膜联蛋白V/PI染色等体外实验数据表明，从体外培养的MCF-7细胞培养基和切除的乳腺癌组织中收集的细胞外因子对HeLa细胞具有诱导凋亡和使细胞周期停滞的作用。在这些体外实验中，我们检测到由于用人乳腺癌细胞的细胞外因子处理，HeLa细胞中高达40%-50%的细胞发生凋亡死亡，并且G2-M期细胞周期阶段从11%增加到25%。讨论与结论：这些观察结果是新颖的，表明乳腺癌的细胞外因子对HeLa细胞具有诱导凋亡和生长抑制作用。这项研究还可以支持癌症恶病质的概念以及关于单个患者罕见的同步发生两种或更多原发性肿瘤可能性的假说。
