利用细菌生产的淬灭体一步法检测流感病毒血凝素。

Single-Step Detection of the Influenza Virus Hemagglutinin Using Bacterially-Produced Quenchbodies.

机构信息

Department of Biological and Chemical Engineering, College of Science and Technology, Hongik University, Sejong-si 30016, Korea.

Key Laboratory of Biological Medicine in Universities of Shandong Province, School of Bioscience and Technology, Weifang Medical University, Weifang 261042, China.

出版信息

Sensors (Basel). 2018 Dec 23;19(1):52. doi: 10.3390/s19010052.

DOI:10.3390/s19010052

PMID:30583603

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6338965/

Abstract

We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

摘要

我们成功地生成了一种 Quenchbody，能够以简单便捷的方式检测流感病毒血凝素 (HA)。通过用 ATTO520 对细菌产生的抗 HA Fab 进行双位点标记，在存在 H1N1 HA 的纳摩尔浓度下，其荧光强度增加了 4.4 倍。我们的结果表明，这种 Quenchbody 有可能作为传感器，用于简单的流感病毒原位检测。