心脏骤停的新分子层面；推动心肺复苏方法
New Molecular Aspects of Cardiac Arrest; Promoting Cardiopulmonary Resuscitation Approaches.
作者信息
Zamanian-Azodi Mona, Rezaei Tavirani Mostafa, Rostami-Nejad Mohammad, Tajik-Rostami Fatemeh
机构信息
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Emerg (Tehran). 2018;6(1):e40. Epub 2018 Jul 2.
INTRODUCTION
Cardiopulmonary resuscitation (CPR) is a method to improve survival of patients with cardiac arrest. This study aimed to identify the key genes affected five minutes after cardiac arrest, hoping to elevate the efficacy of CPR.
METHODS
In this bioinformatics study differentially expressed genes of six pigs were downloaded from GEO and screened. The significant and characterized genes were analyzed via calculating fold change and protein-protein interaction (PPI) networks. The crucial nodes were determined based on centrality parameters and their related biological processes were investigated via ClueGO.
RESULTS
17 significant up-regulated (LogFC ≥ 2) and 22 down-regulated (LogFC < -0.5) genes were detected. Transthyretin (TTR logFC = 4.59) and Gonadotropin-releasing hormone receptor (GNRHR logFC = 3.84) had higher logFC among up-regulated and down-regulated genes, respectively. The critical genes including four up-regulated and five down-regulated genes were detected from network analysis. GNRHR and Prolactin precursor (PRL) were among the most important down res 5 minutes after cardiac arrest and Beta-2 adrenergic receptor and Cadherin-1 were among the most important up regulated gens.
CONCLUSION
The introduced potential biomarkers could reveal a new molecular aspect for CPR performance and pituitary gland protection was highlighted in this respect.
引言
心肺复苏（CPR）是一种提高心脏骤停患者生存率的方法。本研究旨在确定心脏骤停五分钟后受影响的关键基因，以期提高心肺复苏的效果。
方法
在这项生物信息学研究中，从基因表达综合数据库（GEO）下载并筛选了六头猪的差异表达基因。通过计算倍数变化和蛋白质-蛋白质相互作用（PPI）网络对显著且具有特征的基因进行分析。基于中心性参数确定关键节点，并通过ClueGO研究其相关的生物学过程。
结果
检测到17个显著上调（LogFC≥2）和22个下调（LogFC＜-0.5）的基因。在分别上调和下调的基因中，转甲状腺素蛋白（TTR，LogFC = 4.59）和促性腺激素释放激素受体（GNRHR，LogFC = 3.84）具有较高的LogFC值。从网络分析中检测到关键基因，包括四个上调基因和五个下调基因。GNRHR和催乳素前体（PRL）是心脏骤停后5分钟最重要的下调基因，β-2肾上腺素能受体和钙黏蛋白-1是最重要的上调基因。
结论
所引入的潜在生物标志物可为心肺复苏性能揭示新的分子层面，并且在这方面突出了垂体保护。
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