心脏骤停后状态下的化合物-蛋白质相互作用分析
Compound-Protein Interaction Analysis in Condition Following Cardiac Arrest.
作者信息
Zamanian Azodi Mona, Rezaei Tavirani Mostafa, Rezaei Tavirani Majid
机构信息
Student Research Committee, Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Galen Med J. 2018 Dec 31;7:e1380. doi: 10.22086/gmj.v0i0.1380. eCollection 2018.
BACKGROUND
Cardiac arrest (CA) and differentially expressed genes (DEGs) relative to postCA have attracted the attention of scientist to prevent damages, which threaten patients. In the present study, metabolites relevant to DEGs of post-CA condition investigated via protein-compound interaction to understand the pathological mechanisms in the human body.
MATERIALS AND METHODS
STITCH plug-in integrated into Cytoscape V.3.6.1 was used to detect the most significant interacting compounds relative to DEGs of pig's brain after 5 minutes' CA. The genes were obtained from the Gene Expression Omnibus database. The identified elements were considered for further evaluation and validation by literature survey.
RESULT
Findings indicate that biochemical compounds including magnesium, calcium, glucose, glycerol, hydrogen, chloride, sulfate, and estradiol interact with DEGs in the two up- and down-regulated networks.
CONCLUSION
The compounds interacting with DEGs are suitable subjects to analysis for re-regulation of the body after CA.
背景
心脏骤停（CA）以及与心脏骤停后相关的差异表达基因（DEGs）已引起科学家们的关注，以预防对患者构成威胁的损伤。在本研究中，通过蛋白质-化合物相互作用研究了与心脏骤停后状态的差异表达基因相关的代谢物，以了解人体的病理机制。
材料与方法
将STITCH插件集成到Cytoscape V.3.6.1中，用于检测猪脑在心脏骤停5分钟后与差异表达基因最显著相互作用的化合物。这些基因来自基因表达综合数据库。通过文献调查对鉴定出的元素进行进一步评估和验证。
结果
研究结果表明，包括镁、钙、葡萄糖、甘油、氢、氯、硫酸盐和雌二醇在内的生化化合物在两个上调和下调网络中与差异表达基因相互作用。
结论
与差异表达基因相互作用的化合物是分析心脏骤停后身体重新调节的合适研究对象。
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