Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.
J Cell Physiol. 2019 Jul;234(7):11060-11069. doi: 10.1002/jcp.27936. Epub 2018 Dec 24.
The significant consequences of spinal cord injury (SCI) include sensory and motor disability resulting from the death of neuronal cells and axon degeneration. In this respect, overcoming the consequences of SCI including the recovery of sensory and motor functions is considered to be a difficult tasks that requires attention to multiple aspects of treatment. The breakthrough in tissue engineering through the integration of biomaterial scaffolds and stem cells has brought a new hope for the treatment of SCI. In the present study, human endometrial stem cells (hEnSCs) were cultured with human Schwann cells (hSC) in transwells, their differentiation into nerve-like cells was confirmed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) and immunocytochemistry techniques. The differentiated cells (co-hEnSC) were then seeded on the poly ε-caprolactone (PCL)/gelatin scaffolds. The SEM images displayed the favorable seeding and survival of the cells on the scaffolds. The seeded scaffolds were then transplanted into hemisected SCI rats. The growth of neuronal cells was confirmed with immunohistochemical study using NF-H as a neuronal marker. Finally, the Basso, Beattie, and Bresnahan (BBB) test confirmed the recovery of sensory and motor functions. The results suggested that combination therapy using the differentiated hEnSC seeded on PCL/gelatin scaffolds has the potential to heal the injured spinal cord and to limit the secondary damage.
脊髓损伤（SCI）的显著后果包括神经元细胞死亡和轴突退化导致的感觉和运动功能障碍。在这方面，克服 SCI 的后果，包括恢复感觉和运动功能被认为是一项艰巨的任务，需要关注治疗的多个方面。通过生物材料支架和干细胞的整合实现的组织工程的突破为 SCI 的治疗带来了新的希望。在本研究中，将人子宫内膜干细胞（hEnSC）与人雪旺细胞（hSC）共培养于 Transwell 小室中，通过实时定量逆转录聚合酶链反应（qRT-PCR）和免疫细胞化学技术证实其分化为类神经细胞。然后将分化的细胞（共 hEnSC）接种到聚己内酯（PCL）/明胶支架上。SEM 图像显示细胞在支架上良好的接种和存活。然后将接种的支架移植到 SCI 半横断大鼠中。使用 NF-H 作为神经元标志物的免疫组织化学研究证实了神经元细胞的生长。最后，Basso、Beattie 和 Bresnahan（BBB）测试证实了感觉和运动功能的恢复。结果表明，使用 PCL/明胶支架接种分化的 hEnSC 的联合治疗具有治愈损伤脊髓和限制继发性损伤的潜力。
