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碳酸钠辅助和纤维素酶辅助提取的菝葜多糖的理化性质、流变学和热学性质。

Physicochemical, rheological and thermal properties of Mesona chinensis polysaccharides obtained by sodium carbonate assisted and cellulase assisted extraction.

机构信息

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.

出版信息

Int J Biol Macromol. 2019 Apr 1;126:30-36. doi: 10.1016/j.ijbiomac.2018.12.211. Epub 2018 Dec 22.

Abstract

Mesona chinensis has been used as a Chinese folk medicine and main ingredient used to make "black bean jelly" in Asia for hundreds of years. In this study, two polysaccharides (MCP-C and MCP-S) from Mesona chinensis were extracted by using cellulase assisted extraction (CAE) and sodium carbonate assisted extraction (SAE), separately. Then the different physicochemical characteristics, rheological and thermal properties of two polysaccharides were analyzed by ion chromatography, high-performance gel permeation chromatography (HPGPC), Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), rheological and thermogravimetric analysis. Results indicated that the contents of total sugar and uronic acid of MCP-C were higher than MCP-S, while the protein content and molecular weight was lower than that of MCP-S. MCP-S and MCP-C had differences in the surface morphology by SEM, while they all had the typical IR spectra characteristic and amorphous morphology of polysaccharides. The rheological experiments showed that MCP-C and MCP-S exhibited typical pseudoplasticity fluids behavior. The apparent viscosity (η), storage modulus (G') and complex viscosity (η*) of MCP-S were higher than MCP-C. The three-stage decomposition patterns were observed in MCP-C and MCP-S. MCP-S was more stable and had higher initial decomposition temperature (Ti) than MCP-C.

摘要

锦葵作为一种中国民间药物,在亚洲地区已经有几百年的历史,被用作制作“黑凉粉”的主要原料。在这项研究中,我们分别使用纤维素酶辅助提取(CAE)和碳酸钠辅助提取(SAE)从锦葵中提取了两种多糖(MCP-C 和 MCP-S)。然后,我们通过离子色谱、高效凝胶渗透色谱(HPGPC)、傅里叶变换红外光谱(FT-IR)、扫描电子显微镜(SEM)、流变学和热重分析等方法分析了两种多糖的不同理化特性、流变学和热学性质。结果表明,MCP-C 的总糖和糖醛酸含量高于 MCP-S,而蛋白质含量和分子量低于 MCP-S。通过 SEM 观察到 MCP-S 和 MCP-C 的表面形态存在差异,而它们都具有多糖的典型红外光谱特征和无定形形态。流变学实验表明,MCP-C 和 MCP-S 表现出典型的假塑性流体行为。MCP-S 的表观粘度(η)、储能模量(G')和复合粘度(η*)均高于 MCP-C。在 MCP-C 和 MCP-S 中都观察到了三个阶段的分解模式。MCP-S 比 MCP-C 更稳定,初始分解温度(Ti)更高。

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