State Key Laboratory of Materials-Oriented Chemical Engineering, College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
Molecules. 2022 Oct 25;27(21):7209. doi: 10.3390/molecules27217209.
Increasing numbers of exopolysaccharides and their properties have been explored. However, the difficulty of extracting high-viscosity exopolysaccharides has hindered their further industrialization. In this research, we explored a strategy based on encapsulated structure control under different pH to efficiently extract exopolysaccharides (PAPS). Results showed that at pH levels of 6, 12, and 13, the extraction efficiency of PAPS was elevated, and the yield did not decrease. The rheological properties of the pH-12-treated PAPS were better than those of PAPS treated at pH 7, while the pH-6-treated PAPS decreased. The effects of pH-12-treated PAPS on soil macroaggregates and soil's water evaporation rate were similar to those of PAPS treated at pH 7. In addition, we observed that treatment at pH 12 produced a significantly reduced encapsulated structure compared with treatment at pH 7. The proportion of unsaturated fatty acids after treatment at pH 12 was higher than after treatment at pH 7, which may result in reduced encapsulated structure in pH-12 conditions. These results enrich the understanding of the effect that alters pH conditions on the encapsulated structure to improve the extraction efficiency of exopolysaccharides and provide a theoretical basis for the extraction of exopolysaccharides with extreme viscosity.
越来越多的胞外多糖及其性质得到了探索。然而，高粘度胞外多糖提取困难，阻碍了其进一步的工业化应用。在本研究中，我们探索了一种基于不同 pH 值下包裹结构控制的策略，以有效提取胞外多糖（PAPS）。结果表明，在 pH 值为 6、12 和 13 时，PAPS 的提取效率提高，产率没有下降。pH 值为 12 处理的 PAPS 的流变性能优于 pH 值为 7 处理的 PAPS，而 pH 值为 6 处理的 PAPS 则降低了。pH 值为 12 处理的 PAPS 对土壤大团聚体和土壤蒸发速率的影响与 pH 值为 7 处理的 PAPS 相似。此外，我们观察到 pH 值为 12 处理后的包裹结构与 pH 值为 7 处理后的包裹结构相比明显减少。pH 值为 12 处理后的不饱和脂肪酸比例高于 pH 值为 7 处理后的比例，这可能导致 pH 值为 12 条件下包裹结构减少。这些结果丰富了改变 pH 值条件对包裹结构的影响的认识，提高了胞外多糖的提取效率，并为提取具有极端粘度的胞外多糖提供了理论依据。
