Choi Sae Woong, Jeon Seung Hwan, Kwon Eun Bi, Zhu Guan Qun, Lee Kyu Won, Choi Jin Bong, Jeong Hyun Cheol, Kim Kang Sup, Bae Sang Rak, Bae Woong Jin, Kim Su Jin, Cho Hyuk Jin, Ha U Syn, Hong Sung Hoo, Hwang Sung Yeoun, Kim Sae Woong
Department of Urology, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Department of Urology, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea.
World J Mens Health. 2019 Jan;37(1):105-112. doi: 10.5534/wjmh.180051.
Testosterone replacement therapy is an effective treatment for late-onset hypogonadism (LOH) despite a few contraindications and side-effects. The aim of this study was to determine whether modified Ojayeonjonghwan (KH-204, Korean herbal formula) improved LOH. KH-204 is a strong antioxidant herbal formula. We evaluated the effect of Korean herbal prescription on androgen receptor (AR) expression in an aged rat model of LOH.
Eighteen-month-old rats were used as aged LOH rat models. Eighteen Sprague-Dawley rats were randomly divided into three equal groups of six animals each and treated with one of the following: 1) normal control group (oral administration with distilled water, n=6), 2) KH-204 200 group (oral administration with 200 mg/kg of KH-204, n=6), and 3) KH-204 400 group (oral administration with 400 mg/kg of KH-204, n=6). After four weeks of treatment (once daily, distilled water or KH-204), serum testosterone levels, changes in testicular and epididymal weight, Western blotting analysis of AR expression and measurement of oxidative stress were examined.
Treatment with the herbal formulation KH-204 200 mg/kg and 400 mg/kg (1) increased the weights of testis and epididymis; (2) increased the level of serum testosterone; (3) increased the level of superoxide dismutase and reduced the level of 8-hydroxy-20-deoxyguanosine; and (4) upregulated AR expression in testicular tissue.
KH-204 might be an effective alternative for LOH. It improves antioxidant mechanisms and increases testicular AR expression without side-effects.
尽管存在一些禁忌症和副作用，但睾酮替代疗法仍是迟发性性腺功能减退（LOH）的有效治疗方法。本研究的目的是确定改良的奥佳延宗焕（KH - 204，韩国草药配方）是否能改善LOH。KH - 204是一种强效抗氧化草药配方。我们在老年LOH大鼠模型中评估了韩国草药处方对雄激素受体（AR）表达的影响。
18个月大的大鼠用作老年LOH大鼠模型。18只Sprague - Dawley大鼠随机分为三组，每组6只动物，并分别接受以下一种处理：1）正常对照组（口服蒸馏水，n = 6），2）KH - 204 200组（口服200 mg/kg的KH - 204，n = 6），3）KH - 204 400组（口服400 mg/kg的KH - 204，n = 6）。治疗四周后（每天一次，蒸馏水或KH - 204），检测血清睾酮水平、睾丸和附睾重量变化、AR表达的蛋白质印迹分析以及氧化应激测量。
用200 mg/kg和400 mg/kg的草药配方KH - 204治疗（1）增加了睾丸和附睾的重量；（2）提高了血清睾酮水平；（3）提高了超氧化物歧化酶水平并降低了8 - 羟基 - 2'-脱氧鸟苷水平；（4）上调了睾丸组织中AR的表达。
KH - 204可能是治疗LOH的有效替代方法。它改善抗氧化机制，增加睾丸AR表达且无副作用。
