Molecular Plant Biology, Department of Biochemistry, University of Turku, Turku, Finland.
Molecular Genetics of Prokaryotes, University of Freiburg, Freiburg, Germany.
J Appl Microbiol. 2019 Mar;126(3):826-841. doi: 10.1111/jam.14182. Epub 2019 Jan 20.
Cyanobacteria are prokaryotes performing oxygenic photosynthesis, and they can be engineered to harness solar energy for production of commodity and high-value chemicals by means of synthetic biology. The Cu -regulated petJ promoter (P ), which controls the expression of the endogenous cytochrome c553, can be used for expression of foreign products in Synechocystis 6803. We aimed to disclose potential bottlenecks in application of the P in synthetic biology approaches.
Quantitative label-free mass spectrometry revealed global proteome changes which occurred during nutrient conditions which repress or activate of P in Synechocystis 6803.
Some irreversible proteome alterations were discovered due to the copper stress, including downregulation of the ribosomal proteins, significant changes in protein amounts of the cell surface layer and the outer and inner membranes.
This study revealed limitations in the use of P for biotechnological applications.
蓝藻是进行产氧光合作用的原核生物，可通过合成生物学技术对其进行工程改造，以利用太阳能生产大宗商品和高价值化学品。Cu 调控的 petJ 启动子（P ）可以控制内源性细胞色素 c553 的表达，从而在 Synechocystis 6803 中表达外源产物。我们旨在揭示 P 在合成生物学方法中的应用中的潜在瓶颈。
定量无标记质谱分析揭示了在 Synechocystis 6803 中抑制或激活 P 时发生的全蛋白质组变化。
由于铜胁迫，发现了一些不可逆的蛋白质组改变，包括核糖体蛋白的下调、细胞表面层以及内外膜的蛋白质含量的显著变化。
这项研究揭示了 P 在生物技术应用中的局限性。
