Valenčáková Alexandra, Danišová Oľga
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Komenského 73, 04181 Košice, Slovak Republic.
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Komenského 73, 04181 Košice, Slovak Republic.
Acta Trop. 2019 Mar;191:217-220. doi: 10.1016/j.actatropica.2018.12.031. Epub 2018 Dec 23.
Enterocytozoon bieneusi is characterized as a ubiquitous intestinal parasite with a wide genetic diversity, and it is capable of infecting a diverse range of hosts all around the world. Since information about the genotype diversity of E. bieneusi in pigs, calves, sheep and goats in Slovakia is very limited, we examined three farms where we mapped the occurrence of E. bieneusi and its genotypes, thus contributing to the information about geographic diversity of this pathogen worldwide. In this study we used PCR methods to examine 253 fecal samples from pigs, calves, sheep and goats with suspected microsporidiosis. Real time PCR was used to identify genotypes by amplification of SSU region and ITS region. After analysis we detected presence of E. bieneusi (7) and Microsporidia sp. (6) in 13 samples. The analysis of nucleotide sequences of ITS region of E. bieneusi shows, that the positive isolates belonged to 5 genotypes, including two known genotypes (I, F) and three new genotypes diagnosed in pigs, named SVK-S1, SVK-S2 and SVK-S3. Phylogenetic analysis showed that these novel genotypes identified in present study belong to group 1, which previously has been described as a zoonotic group. Genotype I was detected in two calves and genotype F was detected in two pigs.
微小隐孢子虫是一种普遍存在的肠道寄生虫，具有广泛的遗传多样性，能够感染世界各地的多种宿主。由于斯洛伐克猪、犊牛、绵羊和山羊中微小隐孢子虫基因型多样性的信息非常有限，我们对三个农场进行了调查，绘制了微小隐孢子虫的发生情况及其基因型图谱，从而为全球该病原体的地理多样性信息做出了贡献。在本研究中，我们使用PCR方法检测了253份疑似微孢子虫病的猪、犊牛、绵羊和山羊的粪便样本。通过扩增SSU区域和ITS区域，使用实时PCR鉴定基因型。分析后，我们在13个样本中检测到微小隐孢子虫（7份）和微孢子虫属（6份）。微小隐孢子虫ITS区域核苷酸序列分析表明，阳性分离株属于5种基因型，包括两种已知基因型（I、F）和在猪中诊断出的三种新基因型，命名为SVK-S1、SVK-S2和SVK-S3。系统发育分析表明，本研究中鉴定的这些新基因型属于第1组，该组先前被描述为人畜共患病组。在两头犊牛中检测到基因型I，在两头猪中检测到基因型F。
