Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Spitalgasse 23, 1090 Vienna, Austria.
Experimental Laboratory of the Division of Plastic and Reconstructive Surgery, Department of Surgery, Medical University of Vienna, Spitalgasse 23, 1090 Vienna, Austria.
Int J Mol Sci. 2018 Dec 24;20(1):71. doi: 10.3390/ijms20010071.
Fast recovery is crucial for a successful nerve repair and an optimal functional outcome after peripheral nerve injury. Regarding donor site morbidity, autologous transplantation shows great limitations, which urge the need for alternative options in nerve reconstruction. Spider silk was reported as an advantageous material for cell adhesion, migration and proliferation, and its use in conduits is of great interest, especially in combination with cells to improve nerve regeneration. We here described the behavior of a co-culture of human Schwann cells and human adipose-derived stem cells (ADSCs) on spider silk as a new approach. After characterized by immunostaining ADSCs and Schwann cells were seeded in the co-culture on a spider silk scaffold and observed for 21 days. Results showed that cells were attached to the silk and aligned along the silk fibers. With further culture time, cells migrated along the silk and increased in number and formed an almost confluent cell layer. In immunostaining, results suggest that the cell layer was equally composed of ADSCs and Schwann cells. In conclusion, we showed that by providing a guiding structure for directed growth and cells to support nerve regeneration and remyelination, a valid alternative to autologous nerve grafts could have been found.
快速恢复对于周围神经损伤后成功的神经修复和最佳功能结果至关重要。关于供体部位发病率，自体移植显示出很大的局限性，这迫切需要在神经重建中选择替代方案。蜘蛛丝被报道为细胞黏附、迁移和增殖的有利材料，其在导管中的应用引起了极大的兴趣，尤其是与细胞结合以改善神经再生。在这里，我们描述了人雪旺细胞和人脂肪来源干细胞（ADSCs）在蜘蛛丝上共培养的行为，这是一种新方法。通过免疫染色对 ADSCs 和 Schwann 细胞进行特征化后，将其接种在蜘蛛丝支架上的共培养物中，并观察 21 天。结果表明，细胞附着在丝上并沿着丝纤维排列。随着进一步的培养时间，细胞沿着丝迁移，数量增加并形成几乎连续的细胞层。在免疫染色中，结果表明细胞层由 ADSCs 和 Schwann 细胞均匀组成。总之，我们表明，通过提供用于定向生长的引导结构以及支持神经再生和髓鞘形成的细胞，可能找到了一种自体神经移植物的有效替代物。
