University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi 728/II, 389 25 Vodnany, Czech Republic.
Comp Biochem Physiol Part D Genomics Proteomics. 2010 Dec;5(4):302-7. doi: 10.1016/j.cbd.2010.08.003.
Sturgeon and paddlefish (Acipenseriformes), the source of roe consumed as caviar, are a unique and commercially valuable group of ancient fishes. In this study, comparative proteomics was used to analyze protein profiles of spermatozoa from five sturgeon species and one paddlefish: Siberian sturgeon (Acipenser baerii), sterlet (A. ruthenus), Russian sturgeon (A. gueldenstaedtii), starry sturgeon (A. stellatus), beluga (Huso huso), and Mississippi paddlefish (Polyodon spathula). Protein profiles of spermatozoa were determined by isoelectric focusing and two-dimensional electrophoresis (2-DE) high-resolution gels. The peptides, previously selected by 2-DE analysis as potentially species-specific, were obtained by "in-gel" tryptic digestion, followed by matrix-associated laser desorption/ionization time-of-flight/mass spectrometry (MALDI-TOF/MS). Among the 23 protein spots selected, 14 were identified as isoforms of enolase B present in all species, but with different isoelectric points or molecular mass. Exceptions were A. ruthenus and H. huso, species with a close phylogenetic relationship. Glycerol-3-phosphate dehydrogenase was detected exclusively in P. spathula. Phosphoglycerate kinase was detected only in A. ruthenus and H. huso, and 3 additional proteins (fructose bisphosphate aldolase A-2, glycogen phosphorylase type IV and glyceraldehyde-3-phosphate dehydrogenase) were found exclusively in A. gueldenstaedtii and H. huso. This study points to the application of proteomics for differential characterization and comparative studies of acipenseriform species at the molecular level.
鲟鱼和白鲟（鲟形目）是鱼子酱消费的珍贵鱼类，它们是一种独特且具有商业价值的古老鱼类群体。在这项研究中，比较蛋白质组学被用于分析来自 5 种鲟鱼和 1 种白鲟的精子蛋白质图谱：西伯利亚鲟（Acipenser baerii）、小体鲟（A. ruthenus）、俄罗斯鲟（A. gueldenstaedtii）、短吻鲟（A. stellatus）、白鲟（Huso huso）和匙吻鲟（Polyodon spathula）。精子蛋白质图谱通过等电聚焦和二维电泳（2-DE）高分辨率凝胶来确定。通过“胶内”胰蛋白酶消化，先前通过 2-DE 分析选择的肽段，随后进行基质辅助激光解吸/电离飞行时间/质谱（MALDI-TOF/MS）分析。在选择的 23 个蛋白质斑点中，有 14 个被鉴定为存在于所有物种中的烯醇酶 B 的同工型，但等电点或分子量不同。例外的是 A. ruthenus 和 H. huso，它们具有密切的系统发育关系。甘油-3-磷酸脱氢酶仅在 P. spathula 中检测到。磷酸甘油酸激酶仅在 A. ruthenus 和 H. huso 中检测到，另外 3 种蛋白质（果糖二磷酸醛缩酶 A-2、糖原磷酸化酶 IV 和甘油醛-3-磷酸脱氢酶）仅在 A. gueldenstaedtii 和 H. huso 中检测到。这项研究表明，蛋白质组学可用于在分子水平上对鲟形目物种进行差异特征和比较研究。
