Curtis M A, Griffiths G S, Price S J, Coulthurst S K, Johnson N W
MRC Dental Research Unit, London Hospital Medical College, UK.
J Clin Periodontol. 1988 Nov;15(10):628-32. doi: 10.1111/j.1600-051x.1988.tb02263.x.
The total protein concentration of gingival crevicular fluid (GCF), sampled repeatedly over a 10-min period with the minimum of physical irritation to the sulcus, was evaluated in a group of 32 healthy adolescents. The mean concentration of the 1st sample was comparable to that of normal tissue fluids and lymph, irrespective of the state of inflammation of the sample site. However, during repeated sampling, the values rose to resemble serum protein levels, except at those sites with no clinically detectable inflammation. Gel electrophoretic analysis confirmed the increasing proportion of serum-derived molecules in the more proteinaceous GCF samples. The results demonstrate the extreme sensitivity of the gingival vasculature to GCF sampling and consequently the need for accurate standardisation of GCF collection protocols. This will apply particularly when compositional data is to be normalised with respect to the total protein content or when the levels of a serum constituent are being examined.
在一组32名健康青少年中，评估了龈沟液（GCF）的总蛋白浓度。在10分钟内反复采集GCF样本，同时对龈沟造成的物理刺激最小。无论样本部位的炎症状态如何，第一个样本的平均浓度与正常组织液和淋巴液的浓度相当。然而，在重复采样过程中，除了那些临床上未检测到炎症的部位外，其值上升至接近血清蛋白水平。凝胶电泳分析证实，在蛋白质含量更高的GCF样本中，血清来源分子的比例增加。结果表明，牙龈血管系统对GCF采样极其敏感，因此需要对GCF采集方案进行准确标准化。这在根据总蛋白含量对成分数据进行标准化或检查血清成分水平时尤其适用。
