四跨膜蛋白7（TSPAN7）通过上皮-间质转化促进肺癌细胞的迁移和增殖。

TSPAN7 promotes the migration and proliferation of lung cancer cells via epithelial-to-mesenchymal transition.

作者信息

Wang Xianguo, Lin Min, Zhao Jinping, Zhu Shaoping, Xu Ming, Zhou Xuefeng

机构信息

Thoracic and Cardiovascular Surgery, Zhongnan Hospital of Wuhan University, Wuchang District, Wuhan, Hubei 430071, China,

Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

出版信息

Onco Targets Ther. 2018 Dec 13;11:8815-8822. doi: 10.2147/OTT.S167902. eCollection 2018.

DOI:10.2147/OTT.S167902

PMID:30588007

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6300375/

Abstract

PURPOSE

To explore the effects and mechanisms of tetraspanin TSPAN7 on the progression of non-small-cell lung cancer (NSCLC) cells.

PATIENTS AND METHODS

All 125 lung cancer specimens and 60 metastatic tissues were obtained from patients diagnosed with NSCLC, and we used immunohistochemistry to detect the expression of TSPAN7 in NSCLC tissues and adjacent normal tissues. Cell proliferation and invasion ability were determined by MTT, colony formation, and cell migration. The relative protein expression level was analyzed by Western blot analysis.

RESULTS

Our clinical data showed that among 125 patients with lung cancer, TSPAN7 was associated with lymph node status, differentiation, tumor size, and poor prognosis. TSPAN7 knockout inhibited cell proliferation and migration. In addition, TSPAN7 increased the expression of N-cadherin in NSCLC cells by reducing the expression of E-cadherin and vimentin and promoting the cell epithelial-mesenchymal transition (EMT) process. Xenograft transplantation model confirmed the role of TSPAN7 in NSCLC metastasis.

CONCLUSION

TSPAN7-mediated EMT is the key to NSCLC migration. TSPAN7 is a potential target for NSCLC therapy.

摘要

目的

探讨四跨膜蛋白TSPAN7对非小细胞肺癌（NSCLC）细胞进展的影响及机制。

患者与方法

从诊断为NSCLC的患者中获取125份肺癌标本和60份转移组织，采用免疫组织化学法检测NSCLC组织及癌旁正常组织中TSPAN7的表达。通过MTT法、集落形成实验和细胞迁移实验测定细胞增殖和侵袭能力。采用蛋白质免疫印迹分析检测相关蛋白的相对表达水平。

结果

我们的临床数据显示，在125例肺癌患者中，TSPAN7与淋巴结状态、分化程度、肿瘤大小及预后不良相关。TSPAN7基因敲除抑制细胞增殖和迁移。此外，TSPAN7通过降低E-钙黏蛋白和波形蛋白的表达，促进细胞上皮-间质转化（EMT）过程，增加NSCLC细胞中N-钙黏蛋白的表达。异种移植模型证实了TSPAN7在NSCLC转移中的作用。