Qu Chong, Dai Chunmei, Guo Yahua, Qin Rui, Liu Junbao
Department of Neurosurgery, China-Japan Union Hospital of Jilin University, Changchun 130033, Jilin, People's Republic of China.
Department of School Hospital, Changchun University of Chinese Medicine, Changchun 130033, Jilin, People's Republic of China.
Onco Targets Ther. 2018 Dec 19;12:101-111. doi: 10.2147/OTT.S182657. eCollection 2019.
This study aims to investigate the functional role of long noncoding RNA SNHG15 in epithelial ovarian cancer (EOC).
The expression of SNHG15 was measured in EOC cells and tissues using qRT-PCR. The correlation of SNHG15 expression and the clinicopathological characters was statistically analyzed. The prognosis of patients with different clinical features in the high/low SNHG15 expression groups were calculated. Moreover, univariate and multivariate Cox regression analyses were performed to identify the risk factors for poor overall survival (OS) and progression-free survival (PFS). The effect of SNHG15 on the migration and invasion was evaluated using Transwell and Matrigel, respectively. The proliferation ability of EOC cells was tested using colony formation and MTT assay. The influence of SNHG15 on the cisplatin resistance was detected by measuring cell inhibition rate and cell viability.
SNHG15 was upegulated in EOC cells and tissues. High SNHG15 expression was correlated with EOC progression and predicted poor OS and PFS in different subgroups of EOC patients. Moreover, multivariate Cox regression analysis defined high SNHG15 expression as an independent risk factor for poor OS and PFS. Furthermore, functional assays showed that the overexpression of SNHG15 promoted migration and invasion, while the loss of SNHG15 suppressed migration and invasion. Furthermore, the proliferation of EOC cells was improved after the ectopic expression of SNHG15, which was suppressed with SNHG15 deficiency. In addition, cisplatin-resistant EOC cells were established for detecting the effect of SNHG15 on EOC chemoresistance. The results showed that cisplatin-resistant EOC cells exhibited much higher levels of SNHG15 expression than controls, and SNHG15 contributed to the chemoresistance of EOC cells.
This study confirms that SNHG15 contributes to the migration, invasion, proliferation, and chemoresistance of EOC. SNHG15 may serve as a potential therapeutic target and prognostic biomarker of EOC patients.
本研究旨在探讨长链非编码RNA SNHG15在上皮性卵巢癌（EOC）中的功能作用。
采用qRT-PCR检测EOC细胞和组织中SNHG15的表达。对SNHG15表达与临床病理特征的相关性进行统计学分析。计算高/低SNHG15表达组中不同临床特征患者的预后。此外，进行单因素和多因素Cox回归分析，以确定总生存期（OS）和无进展生存期（PFS）差的危险因素。分别使用Transwell和基质胶评估SNHG15对迁移和侵袭的影响。使用集落形成和MTT法检测EOC细胞的增殖能力。通过测量细胞抑制率和细胞活力来检测SNHG15对顺铂耐药性的影响。
SNHG15在EOC细胞和组织中上调。高SNHG15表达与EOC进展相关，并预测EOC患者不同亚组的OS和PFS较差。此外，多因素Cox回归分析将高SNHG15表达定义为OS和PFS差的独立危险因素。此外，功能试验表明，SNHG15的过表达促进迁移和侵袭，而SNHG15的缺失抑制迁移和侵袭。此外，SNHG15异位表达后EOC细胞的增殖得到改善，而SNHG15缺乏则抑制了增殖。此外，建立了顺铂耐药的EOC细胞以检测SNHG15对EOC化疗耐药性的影响。结果表明，顺铂耐药的EOC细胞中SNHG15表达水平远高于对照组，且SNHG15有助于EOC细胞的化疗耐药性。
本研究证实SNHG15促进EOC的迁移、侵袭、增殖和化疗耐药性。SNHG15可能作为EOC患者潜在的治疗靶点和预后生物标志物。
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