Department of Oncology, The Second Xiangya Hospital of Central South University, Changsha, 410000, Hunan, China.
BMC Cancer. 2020 Nov 26;20(1):1156. doi: 10.1186/s12885-020-07649-9.
The snoRNA host gene SNHG15 produces a long non-coding RNA (lncRNA) with a short half-life and has been reported to be dysregulated in multiple cancers and has recently been found to be correlated with tumour progression. Therefore, this meta-analysis was performed to evaluate the generalised prognostic role of small nucleolar RNA host gene 15 (SNHG15) in malignancies, based on variable data from different studies.
Four public databases were used to identify eligible studies. The association between prognostic indicators and clinical features was extracted and pooled to estimate the hazard ratios (HRs) or odds ratios (ORs) with 95% confidence intervals (CIs). Publication bias was measured using Begg's test and Egger's test, and the stability of pooled results were measured using sensitivity analysis. Additionally, an online database based on The Cancer Genome Atlas (TCGA) was screened to further validate our results. Ultimately, we predicted the molecular regulation of SNHG15 based on the public databases.
In total, 11 studies including 1087 patients were ultimately enrolled in our meta-analysis. We found that SNHG15 overexpression was associated with worse overall survival (OS) and disease-free survival (DFS), and this was validated in the Gene Expression Profiling Interactive Analysis (GEPIA) cohort. Moreover, increased SNHG15 expression suggested advanced TNM stage and LNM, but was not associated with age, gender, or tumour size. No publication bias or instability of the results was observed. SNHG15 was significantly upregulated in seven cancers and elevated expression of SNHG15 indicated shorter OS and DFS in five malignancies based on the validation using the GEPIA cohort. Further functional prediction indicated that SNHG15 may participate in some cancer-related pathways.
Upregulation of lncRNA SNHG15 was notably associated with worse prognosis and clinical features, suggesting that SNHG15 might serve as a novel prognostic factor in various cancers.
snoRNA 宿主基因 SNHG15 产生具有短半衰期的长非编码 RNA（lncRNA），已在多种癌症中被报道失调，最近发现与肿瘤进展相关。因此，本荟萃分析基于来自不同研究的可变数据，评估小核仁 RNA 宿主基因 15（SNHG15）在恶性肿瘤中的一般预后作用。
使用四个公共数据库来确定合格的研究。提取并汇总与预后指标和临床特征相关的关联，以估计风险比（HR）或优势比（OR）及其 95%置信区间（CI）。使用 Begg 检验和 Egger 检验测量发表偏倚，并使用敏感性分析测量汇总结果的稳定性。此外，还筛选了基于癌症基因组图谱（TCGA）的在线数据库以进一步验证我们的结果。最终，我们根据公共数据库预测了 SNHG15 的分子调控。
总共纳入了 11 项研究，共计 1087 名患者。我们发现 SNHG15 过表达与总生存期（OS）和无病生存期（DFS）更差相关，这在基因表达谱交互分析（GEPIA）队列中得到了验证。此外，SNHG15 表达增加提示 TNM 分期和 LNM 较晚，但与年龄、性别或肿瘤大小无关。未观察到发表偏倚或结果不稳定。SNHG15 在七种癌症中显著上调，基于 GEPIA 队列的验证，SNHG15 表达升高表明五种恶性肿瘤的 OS 和 DFS 更短。进一步的功能预测表明，SNHG15 可能参与了一些癌症相关途径。
lncRNA SNHG15 的上调与预后和临床特征明显相关，表明 SNHG15 可能是各种癌症中的一种新的预后因素。
