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二维有序生物结构电子显微照片的计算机图像处理介绍

Introduction to the computer image processing of electron micrographs of two-dimensionally ordered biological structures.

作者信息

Stewart M

机构信息

Medical Research Council, Laboratory of Molecular Biology, Cambridge, England.

出版信息

J Electron Microsc Tech. 1988 Aug;9(4):301-24. doi: 10.1002/jemt.1060090403.

Abstract

Methods are described for the analysis of electron micrographs of regular biological objects. Fourier-based processing of one-dimensionally ordered arrays is described by way of introduction, before analysing two-dimensional crystals in projection with the aim of enhancing signal:noise ratio and thus of feint features that were initially obscured. This form of analysis is then extended to decomposing the moiré patterns formed when sheets overlap, thereby enabling the separation of interfering image patterns. Analogous forms of an analysis can also be applied to objects with rotational symmetry. Methods for treating the effect of the microscope imaging system and compensating for lattice disorder in crystalline specimens are also discussed.

摘要

本文描述了分析规则生物物体电子显微镜图像的方法。在分析二维晶体投影以提高信噪比从而增强最初模糊不清的微弱特征之前,先通过介绍基于傅里叶变换的一维有序阵列处理方法。然后将这种分析形式扩展到分解薄片重叠时形成的莫尔条纹图案,从而实现干扰图像图案的分离。类似的分析形式也可应用于具有旋转对称性的物体。本文还讨论了处理显微镜成像系统的影响以及补偿晶体标本中晶格无序的方法。

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