人胸腺上皮细胞上清液对人淋巴细胞的激活作用。
Activation of human lymphocytes by supernatants from human thymic epithelium.
作者信息
Goust J M, Vesole D H, Fudenberg H H
出版信息
Clin Exp Immunol. 1979 Nov;38(2):348-55.
Supernatants from human thymic epithelial cells (TS) were found to have a mitogenic effect on cultured human peripheral blood mononuclear cells and to potentiate their responses to lectins. This was not observed with culture supernatants from the human cell lines AV-3 and HeLa or from the murine cell line L-929. The maximum potentiating effects were observed with pokeweed mitogen (PWM) and phytohaemagglutinin (PHA), whereas the response to concanavalin A (Con A) was only slightly enhanced. TS also potentiated the mixed lymphocyte culture (MLC) response of normal T cells and thymocytes cultured with mitomycin C-treated B lymphoid cell lines. The mitogenic effect of TS was time-dependent and paralleled the appearance of lymphoid colonies in semi-solid agar. Chromatographical separation of concentrated serum-free TS on Sephadex G-100 yielded an active fraction of molecular weight 15,000--25,000 which had all the activities of unseparated TS.
研究发现，人胸腺上皮细胞（TS）的上清液对培养的人外周血单个核细胞具有促有丝分裂作用，并能增强其对凝集素的反应。人细胞系AV-3和HeLa以及鼠细胞系L-929的培养上清液则未观察到这种现象。用商陆有丝分裂原（PWM）和植物血凝素（PHA）时观察到最大的增强作用，而对刀豆球蛋白A（Con A）的反应仅略有增强。TS还增强了用丝裂霉素C处理的B淋巴母细胞系培养的正常T细胞和胸腺细胞的混合淋巴细胞培养（MLC）反应。TS的促有丝分裂作用具有时间依赖性，并且与半固体琼脂中淋巴样集落的出现平行。在Sephadex G-100上对浓缩的无血清TS进行色谱分离，得到了分子量为15,000 - 25,000的活性部分，其具有未分离TS的所有活性。
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