Centre for Materials and Surface Science and Department of Chemistry and Physics , La Trobe University , Melbourne , Victoria 3086 , Australia.
CSIRO Manufacturing , Bayview Avenue, Clayton , Vic 3168 , Australia.
Biomacromolecules. 2019 Feb 11;20(2):813-825. doi: 10.1021/acs.biomac.8b01427. Epub 2019 Jan 9.
Electrospun ultrafine fibers prepared using a blend of poly(lactide- co-glycolide) (PLGA) and bromine terminated poly(l-lactide) (PLA-Br), were surface modified using surface-initiated (SI) Cu(0) mediated polymerization. Copolymers based on N-acryloxysuccinimide (NAS) and a low fouling monomer (either N, N-dimethylacrylamide (DMA), N-(2-hydroxypropyl)acrylamide (HPA), or N-acryloylmorpholine (NAM)) were grafted from the fiber surface to impart surface functionality and to reduce nonspecific protein adsorption. Inclusion of the functional NAS monomer facilitated the conjugation of a nonbioactive cyclic RAD peptide and a bioactive cyclic RGD peptide, the latter expected to facilitate cell adhesion through its affinity for the αβ integrin receptor. A detailed analysis of the surface of the electrospun fiber scaffolds in nongrafted form compared to the surface functionalized state is presented. Characteristic amino acid peaks are observed for both conjugated RGD and RAD peptides. Cell culture experiments confirmed cell specific attachment mediated through the presence of the bioactive RGD peptide mainly at high surface density.
采用聚（丙交酯-共-乙交酯）（PLGA）和溴封端聚（L-丙交酯）（PLA-Br）共混物制备的静电纺超细纤维，通过表面引发（SI）Cu（0）介导的聚合进行表面改性。基于 N-丙烯酰氧基琥珀酰亚胺（NAS）和低污染单体（N，N-二甲基丙烯酰胺（DMA）、N-（2-羟丙基）丙烯酰胺（HPA）或 N-丙烯酰吗啉（NAM））的共聚物从纤维表面接枝，赋予表面功能并减少非特异性蛋白质吸附。功能性 NAS 单体的包含促进了非活性环状 RAD 肽和活性环状 RGD 肽的缀合，后者通过与其 αβ 整合素受体的亲和力促进细胞黏附。对未接枝形式的静电纺纤维支架表面与表面功能化状态的详细分析表明。观察到两种缀合的 RGD 和 RAD 肽的特征氨基酸峰。细胞培养实验证实，通过存在生物活性 RGD 肽主要在高表面密度下介导细胞特异性附着。
