超氧化物歧化酶、二硫苏糖醇和甲酸根离子对通气溶液中羟基自由基和超氧阴离子自由基使木瓜蛋白酶失活的影响。
Effects of superoxide dismutase, dithiothreitol and formate ion on the inactivation of papain by hydroxyl and superoxide radicals in aerated solutions.
作者信息
Lin W S, Armstrong D A, Lal M
出版信息
Int J Radiat Biol Relat Stud Phys Chem Med. 1978 Mar;33(3):231-43. doi: 10.1080/09553007814550121.
Losses in enzyme activity and sulphydryl content have been studied in aerated papain solutions containing formate, superoxide dismutase and dithiothreitol. Both formate and dithiothreitol converted .OH to .O2-, whereas superoxide dismutase completely suppressed the inactivation by .O2-. Using results from all three systems, the fraction of .O2- reactions with papain that caused inactivation of the enzyme was 0.33 +/- 0.07. The results also showed that the fraction of .OH reactions, which cause inactivation of papain, is significantly higher in aerated than in oxygen-free solutions.
已对含有甲酸盐、超氧化物歧化酶和二硫苏糖醇的通气木瓜蛋白酶溶液中的酶活性损失和巯基含量进行了研究。甲酸盐和二硫苏糖醇都将·OH转化为·O₂⁻，而超氧化物歧化酶完全抑制了·O₂⁻引起的失活。利用这三个系统的结果，与木瓜蛋白酶发生反应并导致该酶失活的·O₂⁻的比例为0.33±0.07。结果还表明，导致木瓜蛋白酶失活的·OH反应比例在通气溶液中显著高于无氧溶液。
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