Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-Agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Wetlands, Yancheng Teachers University, Yancheng, 224001, Jiangsu, China.
College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211800, Jiangsu, China.
Mol Biol Rep. 2021 Mar;48(3):2611-2618. doi: 10.1007/s11033-021-06315-y. Epub 2021 Apr 3.
Procambarus clarkii is an important model crustacean organism in many researches. Ammonia nitrogen is one of common contaminants in aquatic environment, influencing the health of aquatic organisms. The primary objective of this study was to investigate molecular mechanisms on ammonia stress in gills of P. clarkii to provide new insights into the strategies of aquatic animals in responding to high concentration of ammonia in the environment. Procambarus clarkii were randomly assigned into two groups (ammonia stress group, AG; control group, CG), and gill samples were dependently excised from AG and CG. Then response mechanisms on ammonia stress were investigated based on transcriptome data of P. clarkii. 9237 differentially expressed genes were identified in ammonia stress group. The genes of ion transport enzymes (NKA and SLC6A5S) were significantly up-regulated. Whereas the immune-related genes (e.g. MAP3K7, HSP70, HSP90A, CTSF, CTSL1, CHI and CTL4) and pathways were significantly up-regulated, which played an important role in reacting to ammonia stress. Procambarus clarkii may enhance immune defense to counteract ammonia toxicity by the up-regulation of immune-related genes and signaling pathways. The activities of ion transport enzymes are changed to mobilise signal transduction and ion channel regulation for adapting to ammonia environment. These previous key genes play an important role in resistance to ammonia stress to better prepare for survival in high concentration of ammonia.
克氏原螯虾是许多研究中的一种重要模式甲壳类生物。氨氮是水生环境中的常见污染物之一，会影响水生生物的健康。本研究的主要目的是研究克氏原螯虾鳃中对氨胁迫的分子机制，为水生动物应对环境中高浓度氨的策略提供新的见解。将克氏原螯虾随机分为两组（氨胁迫组，AG；对照组，CG），并从 AG 和 CG 中独立取出鳃样本。然后基于克氏原螯虾的转录组数据研究对氨胁迫的反应机制。在氨胁迫组中鉴定出 9237 个差异表达基因。离子转运酶（NKA 和 SLC6A5S）的基因显著上调。而免疫相关基因（如 MAP3K7、HSP70、HSP90A、CTSF、CTSL1、CHI 和 CTL4）和途径显著上调，在应对氨胁迫中发挥重要作用。克氏原螯虾可能通过上调免疫相关基因和信号通路来增强免疫防御，以对抗氨毒性。离子转运酶的活性发生变化，以调动信号转导和离子通道调节，以适应氨环境。这些先前的关键基因在抵抗氨胁迫中发挥重要作用，以更好地为在高浓度氨中生存做好准备。
