Cohen J S
Medicine Branch, National Cancer Institute, Bethesda, MD 20892.
Mayo Clin Proc. 1988 Dec;63(12):1199-207. doi: 10.1016/s0025-6196(12)65406-8.
31P magnetic resonance spectroscopy (MRS) has been established as an excellent noninvasive procedure for studying metabolism in cancer cells. For a full understanding of the results of in vivo MRS studies of metabolism, it is necessary to be able to conduct controlled experiments on the same intact functioning cells in a perfusion apparatus. The specific techniques used for this purpose are described, and the applications to cancer cell bioenergetics and phospholipid metabolism in drug-resistant and drug-sensitive breast cancer cells are illustrated. Spectra of the same cell line grown in a "model tumor" in natural basement membrane gel and in nude mice in vivo are compared. The results indicate a potential for the use of 31P MRS in a clinical setting for monitoring therapy and for diagnosis.
31P磁共振波谱（MRS）已成为研究癌细胞代谢的一种出色的非侵入性方法。为了全面理解体内MRS代谢研究的结果，有必要能够在灌注装置中对相同完整功能的细胞进行对照实验。描述了用于此目的的具体技术，并举例说明了其在耐药和药物敏感乳腺癌细胞的癌细胞生物能量学和磷脂代谢中的应用。比较了在天然基底膜凝胶中的“模型肿瘤”和体内裸鼠中生长的同一细胞系的波谱。结果表明31P MRS在临床环境中用于监测治疗和诊断的潜力。
