Bone Tumour and Bone Disease Department II, Zhengzhou Orthopaedic Hospital, Zhengzhou, China.
College of Safety Science and Engineering, Henan Polytechnic University, Jiaozuo, China.
J Mol Recognit. 2019 Jun;32(6):e2775. doi: 10.1002/jmr.2775. Epub 2018 Dec 28.
Combining the surface modification and molecular imprinting technique, a novel piezoelectric sensing platform with excellent molecular recognition capability was established for the detection of uric acid (UA) based on the immobilization of TiO nanoparticles onto quartz crystal microbalance (QCM) electrode and modification of molecularly imprinted TiO (MIT) layer on TiO nanoparticles. The performance of the fabricated biosensor was evaluated, and the results indicated that the biosensor exhibited high sensitivity in UA detection, with a linear range from 0.04 to 45 μM and a limit of detection of 0.01 μM. Moreover, the biosensor presented high selectivity towards UA in comparison with other interferents. The analytical application of the UA biosensor confirmed the feasibility of UA detection in urine sample.
结合表面修饰和分子印迹技术，通过将 TiO2 纳米粒子固定在石英晶体微天平（QCM）电极上，并在 TiO2 纳米粒子上修饰分子印迹 TiO2（MIT）层，构建了一种具有优异分子识别能力的新型压电传感平台，用于尿酸（UA）的检测。对所制备的生物传感器的性能进行了评估，结果表明，该生物传感器在 UA 检测中表现出高灵敏度，线性范围为 0.04 至 45 μM，检测限为 0.01 μM。此外，与其他干扰物相比，该生物传感器对 UA 具有较高的选择性。UA 生物传感器的分析应用证实了在尿液样本中检测 UA 的可行性。
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