van der Zwan J C, Dankert J, de Vries K, Orie N G, Kauffman H F
J Clin Pathol. 1978 Apr;31(4):370-7. doi: 10.1136/jcp.31.4.370.
After purifying a Haemophilus influenzae precipitinogen from endotoxic activity by means of ultracentrifugation, column chromatography (Sepharose 6B) and ion exchange chromatography (DEAE Sephadex A25) a fraction was obtained which still contained a specific precipitinogen that was virtually free of endotoxin. Furthermore, during the chromatographic procedures fractions with a high and a low molecular weight endotoxic activity were found. The limulus lysate test was more sensitive in the high molecular weight fractions and the LD50 in mice in the low molecular weight fractions with endotoxic activity.
通过超速离心、柱色谱法（琼脂糖6B）和离子交换色谱法（DEAE葡聚糖A25）从内毒素活性中纯化流感嗜血杆菌沉淀原后，获得了一个仍含有几乎不含内毒素的特异性沉淀原的级分。此外，在色谱过程中发现了具有高分子量和低分子量内毒素活性的级分。鲎试剂检测对高分子量级分更敏感，而低分子量具有内毒素活性的级分对小鼠的半数致死剂量（LD50）。
