Raichvarg D, Guenounou M, Brossard C, Agneray J, Alouf J E
Med Microbiol Immunol. 1980;168(3):201-10. doi: 10.1007/BF02122854.
Two preparations designated as PWE and NCE were obtained from H, influenzae type a by treatment with either phenol water or hypertonic NaCl citrate respectively. The protein content of NCE was three fold higher than for PWE. Electroimmunodiffusion against H. influenzae antiserum disclosed an immunological relationship between NCE and PWE. The toxicity for mice was lower for both antigenic extracts (LD50 PWE: 47 +/- 7 mg/kg; LD50 NCE: 943 +/- 5 mg/kg) than for E. coli LPS. Limulus assay showed that both H. influenzae extracts had a weaker potency (100 to 1000 times less) than E. coli LPS. Sanarelli and Shwartzman reactions failed for PWE and NCE. The blast transformation of DBA/2 and nude mouse spleen cells were strikingly different for the three preparations: PWE, NCE, and E. coli LPS. Neither extract from H. influenzae cells showed the biological properties of a classical endotoxin. We suggest that they are endotoxin-like substances.
分别用酚水或高渗氯化钠柠檬酸盐处理甲型流感嗜血杆菌，得到了两种制剂，分别命名为PWE和NCE。NCE的蛋白质含量比PWE高三倍。用流感嗜血杆菌抗血清进行电免疫扩散显示，NCE和PWE之间存在免疫关系。两种抗原提取物对小鼠的毒性均低于大肠杆菌脂多糖（PWE的半数致死量：47±7毫克/千克；NCE的半数致死量：943±5毫克/千克）。鲎试剂检测表明，两种流感嗜血杆菌提取物的效力均比大肠杆菌脂多糖弱（低100至1000倍）。PWE和NCE均未出现桑纳雷利反应和施瓦茨曼反应。三种制剂（PWE、NCE和大肠杆菌脂多糖）对DBA/2和裸鼠脾细胞的增殖转化作用显著不同。流感嗜血杆菌细胞的两种提取物均未表现出典型内毒素的生物学特性。我们认为它们是类内毒素物质。
