通过水解酶分离和纯化内毒素。
Isolation and purification of endotoxin by hydrolytic enzymes.
作者信息
Lehrer S, Nowotny A
出版信息
Infect Immun. 1972 Dec;6(6):928-33. doi: 10.1128/iai.6.6.928-933.1972.
Abstract
Various commercial hydrolases were used in an attempt to degrade the endotoxic lipopolysaccharide macromolecule. Some inert components, such as peptides and nucleic acids, could be removed from endotoxin preparations. As a result, endotoxic activity, measured by pyrogenicity, Shwartzman reaction, and mouse lethality, was increased. The remarkable resistance of endotoxin to hydrolases led to the use of such enzymes for the liberation and purification of endotoxin from whole bacterial cells.
摘要
为了降解内毒素脂多糖大分子，人们使用了多种商业水解酶。一些惰性成分，如肽和核酸，可以从内毒素制剂中去除。结果，通过热原性、施瓦茨曼反应和小鼠致死率测量的内毒素活性增加了。内毒素对水解酶具有显著的抗性，这导致人们使用此类酶从全细菌细胞中释放和纯化内毒素。
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