Xu Lin, Fan Chun-Lin, Huang Xiao-Jun, Zeng Hu-Hu, Liu Hui, Wang Ying, Ye Wen-Cai
Institute of Traditional Chinese Medicine & Natural Products, College of Pharmacy, Jinan University, Guangzhou 510632, China.
Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine and New Drugs Research, Jinan University, Guangzhou 510632, China.
Zhongguo Zhong Yao Za Zhi. 2018 Nov;43(22):4474-4478. doi: 10.19540/j.cnki.cjcmm.20180820.006.
This study aims to establish the characteristic fingerprint of the leaves of Moringa oleifera by Ultra High Performance Liquid Chromatography (UPLC) for its quality control. The method was developed on a column of Agilent Eclipse XDB-C₁₈ with acetonitrile-0.01% TFA solution as the mobile phase by gradient elution at a flow rate of 0.5 mL·min⁻¹. The detective wavelength was 210 nm, and the column temperature was 35 °C. The 14 batches of the leaves of M. oleifera were compared for the similarity by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (2004A). The UPLC characteristic fingerprint was established, and twelve common peaks were identified by comparison with the references and UPLC-MS. The relative retention times were 0.08 (No. 1, adenosine), 0.14 (No. 2, L-phenylalanine), 0.22 (No. 3, 5-caffeoylquinic acid), 0.28 (No. 4, L-tryptophane), 0.42 (No. 5, 4-caffeoylquinic acid), 0.65 (No. 6, vicenin-2), 0.94 (No. 7, vitexin), 0.96 (No. 8, isovitexin), 1.00 (No. 9, isoquercitrin), 1.11 [No. 10, quercetin 3-O-β-D-(6"-malonyl)-glucopyranoside], 1.21 (No. 11, astragalin) and 1.37 [No. 12, kaempferol 3-O-β-D-(6"-malonyl)-glucopyranoside]. It is the first time to establish the UPLC characteristic fingerprint of the leaves of M. oleifera. The method is simple, quick and reproducible with high precision, which can provide a scientific basis for the quality control of the leaves of M. oleifera.
本研究旨在通过超高效液相色谱法（UPLC）建立辣木叶的特征指纹图谱，用于其质量控制。该方法采用安捷伦Eclipse XDB-C₁₈色谱柱，以乙腈-0.01%三氟乙酸溶液为流动相，梯度洗脱，流速为0.5 mL·min⁻¹。检测波长为210 nm，柱温为35℃。采用《中药色谱指纹图谱相似度评价系统》（2004A版）对14批辣木叶进行相似度比较。建立了UPLC特征指纹图谱，并通过与对照品及UPLC-MS比较鉴定出12个共有峰。相对保留时间分别为0.08（峰1，腺苷）、0.14（峰2，L-苯丙氨酸）、0.22（峰3，5-咖啡酰奎宁酸）、0.28（峰4，L-色氨酸）、0.42（峰5，4-咖啡酰奎宁酸）、0.65（峰6，异荭草苷）、0.94（峰7，牡荆素）、0.96（峰8，异牡荆素）、1.00（峰9，异槲皮苷）、1.11[峰10，槲皮素3-O-β-D-(6″-丙二酰基)-葡萄糖苷]、1.21（峰11，紫云英苷）和1.37[峰12，山柰酚3-O-β-D-(6″-丙二酰基)-葡萄糖苷]。首次建立了辣木叶的UPLC特征指纹图谱。该方法简便、快速、重现性好、精密度高，可为辣木叶的质量控制提供科学依据。
