使用蛋白酶K保护试验检测丙型肝炎病毒包膜化
Measuring Hepatitis C Virus Envelopment by Using a Proteinase K Protection Assay.
作者信息
Roder Allison E, Horner Stacy M
机构信息
Department of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC, USA.
Department of Medicine, Duke University Medical Center, Durham, NC, USA.
出版信息
Methods Mol Biol. 2019;1911:209-217. doi: 10.1007/978-1-4939-8976-8_14.
The infectious virion of hepatitis C virus (HCV) is made up of the viral nucleocapsid surrounded by an envelope that contains an ER-derived membrane bilayer, cellular lipids, and the viral E1 and E2 glycoproteins. Because the infectious HCV particle contains both protein and lipid layers, selective disruption of these layers and analysis for the presence or absence of resulting virion components can be used to study the virion assembly process. This chapter describes an experimental method to measure HCV virion envelopment, which can reveal the mechanisms of how specific viral protein-protein interactions and host factors contribute to the process of HCV envelopment.
丙型肝炎病毒（HCV）的感染性病毒粒子由被包膜包围的病毒核衣壳组成，该包膜包含源自内质网的膜双层、细胞脂质以及病毒E1和E2糖蛋白。由于感染性HCV粒子同时包含蛋白质层和脂质层，对这些层进行选择性破坏并分析所得病毒粒子成分的存在与否，可用于研究病毒粒子的组装过程。本章介绍一种测量HCV病毒粒子包膜化的实验方法，该方法可揭示特定病毒蛋白-蛋白相互作用和宿主因子如何促进HCV包膜化过程的机制。
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