Temel Münevver Tugba, Demiryürek Seniz, Temel Levent, Saracaloglu Ahmet, Eke Necmi, Baysalman Elif, Mammadov Azad, Coskun Mehmet E, Demiryürek Abdullah T
Department of Pediatrics, University of Gaziantep, Gaziantep, Turkey.
Department of Physiology, University of Gaziantep, Gaziantep, Turkey.
Pediatr Int. 2019 Mar;61(3):252-257. doi: 10.1111/ped.13773. Epub 2019 Feb 22.
Alteration in thiol level under oxidative stress may contribute to community-acquired pneumonia (CAP). The goal of this study was to determine whether there are changes in thiol/disulfide homeostasis and nitric oxide (NO) in children with CAP.
In total, 130 participants were involved in the study. Of these, 65 had been diagnosed with CAP on admission, and the remaining 65 were healthy individuals. Serum total thiol and native thiol were measured in each participant using a novel automated spectrophotometric method. The amount of dynamic disulfide bonds and related ratios were calculated from these values. Serum NO was measured on chemiluminescence assay.
Average native thiol, total thiol, and disulfide in the CAP group were significantly lower than in the healthy individuals (P < 0.0001, P < 0.0001, P = 0.0126, respectively). In addition, disulfide/native thiol (P = 0.0002), and disulfide/total thiol ratios (P = 0.0004) were significantly higher, whereas the native thiol/total thiol ratio (P = 0.0004) was lower in the CAP group. High serum NO was noted in the CAP group (P = 0.0003), but there was no marked correlation between thiol/disulfide and NO.
The changes in endogenous thiol levels under oxidative stress may be associated with the pathogenesis of CAP in pediatric patients.
氧化应激下硫醇水平的改变可能与社区获得性肺炎（CAP）有关。本研究的目的是确定CAP患儿的硫醇/二硫键稳态和一氧化氮（NO）是否存在变化。
共有130名参与者纳入本研究。其中，65名入院时被诊断为CAP，其余65名是健康个体。采用一种新型自动分光光度法测定每位参与者的血清总硫醇和天然硫醇。根据这些值计算动态二硫键的量和相关比率。采用化学发光法测定血清NO。
CAP组的平均天然硫醇、总硫醇和二硫键水平显著低于健康个体（分别为P < 0.0001、P < 0.0001、P = 0.0126）。此外，CAP组的二硫键/天然硫醇比率（P = 0.0002）和二硫键/总硫醇比率（P = 0.0004）显著更高，而天然硫醇/总硫醇比率更低（P = 0.0004）。CAP组血清NO水平较高（P = 0.0003），但硫醇/二硫键与NO之间无明显相关性。
氧化应激下内源性硫醇水平的变化可能与小儿CAP的发病机制有关。
