Department of Pediatrics, Faculty of Medicine, Sakarya University, Sakarya, Turkey.
Department of Biochemistry, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey.
Neurol Sci. 2017 Nov;38(11):1969-1975. doi: 10.1007/s10072-017-3087-2. Epub 2017 Aug 14.
Simple febrile seizures are generally benign, but during the seizure, elevated levels of glutamate and high levels of oxygen use due to the high metabolic brain activity result in oxidative stress. However, the relationship between febrile seizures and oxidative stress remains unclear. In this study, we investigated thiol/disulfide homeostasis as a new oxidative stress parameter in patients with simple febrile seizures. This study was performed between February 2016 and May 2016 at the Pediatric Emergency Unit. The study population consisted of 40 patients with a diagnosis of simple febrile seizure and 30 control participants aged 8-59 months. Total thiol, native thiol and disulfide levels, disulfide/native thiol, disulfide/total thiol, and native thiol/total thiol ratios were used as thiol/disulfide homeostasis parameters and were quantified in patient and control groups. Furthermore, correlations with seizure duration were investigated. In the patient group, native and total thiol levels and native thiol/total thiol ratios were low, and disulfide levels, disulfide/native thiol, and disulfide/total thiol ratios were significantly higher than in the control group. Negative correlations were observed between seizure duration, total and native thiol levels, and native thiol/total thiol ratio, whereas positive correlations were observed between seizure duration and disulfide/native thiol and disulfide/total thiol ratio. The sensitivities of both disulfide/native thiol and disulfide/total thiol ratios were high for simple febrile seizures. Simple febrile seizures may cause impairment in favor of disulfide bonds in thiol/disulfide homeostasis. Overall, these changes may contribute to neuronal cell damage after simple febrile seizures.
单纯性热性惊厥通常是良性的，但在惊厥期间，由于大脑代谢活动高，谷氨酸水平升高和耗氧量增加，导致氧化应激。然而，热性惊厥与氧化应激之间的关系尚不清楚。在这项研究中，我们研究了巯基/二硫键平衡作为单纯性热性惊厥患者的新氧化应激参数。该研究于 2016 年 2 月至 2016 年 5 月在儿科急诊室进行。研究人群包括 40 例单纯性热性惊厥患者和 30 名年龄在 8-59 个月的对照组参与者。总巯基、天然巯基和二硫键水平、二硫键/天然巯基、二硫键/总巯基和天然巯基/总巯基比作为巯基/二硫键平衡参数在患者组和对照组中进行了定量分析。此外，还研究了与惊厥持续时间的相关性。在患者组中，天然和总巯基水平以及天然巯基/总巯基比值较低，而二硫键水平、二硫键/天然巯基和二硫键/总巯基比值显著高于对照组。惊厥持续时间与总巯基和天然巯基水平以及天然巯基/总巯基比值呈负相关，而与二硫键/天然巯基和二硫键/总巯基比值呈正相关。二硫键/天然巯基和二硫键/总巯基比值对单纯性热性惊厥的敏感性均较高。单纯性热性惊厥可能导致巯基/二硫键平衡中有利于二硫键的损伤。总的来说，这些变化可能导致单纯性热性惊厥后神经元细胞损伤。
