气体调节微流控装置中的缺氧生理环境

Hypoxic Physiological Environments in a Gas-Regulated Microfluidic Device.

作者信息

Lee Insu, Woo Jin Hyuk, Lee Min, Jeon Tae-Joon, Kim Sun Min

机构信息

Department of Mechanical Engineering, Inha University, Incheon 22212, Korea.

Division of Advanced Prosthodontics, University of California at Los Angeles, 10833 Le Conte Avenue, Los Angeles, CA 90095, USA.

出版信息

Micromachines (Basel). 2018 Dec 28;10(1):16. doi: 10.3390/mi10010016.

DOI:10.3390/mi10010016

PMID:30597832

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6356689/

Abstract

Hypoxic environment is known as one of the critical factors in various physiological/pathological processes. It is imperative to recapitulate oxygen level in microscale for human physiology/pathology induced by hypoxia. Herein, we propose an oxygen-regulating system that can be applied to in vitro tissue models. We fabricated a microdevice with a gas-permeable membrane, allowing oxygen diffusion without direct contact to cells. We verified the formation of oxygen level less than 2% O₂ concentration inside the device through computational simulation and experiments. H9c2 heart myoblasts were exposed to hypoxic condition in the device, and their cell viability were investigated. We anticipate that our system will be integrated with a platform to study hypoxia-induced human physiology and pathology as an efficient oxygen-regulating system.

摘要

缺氧环境是各种生理/病理过程中的关键因素之一。在微观尺度上重现由缺氧引起的人类生理/病理过程中的氧水平势在必行。在此，我们提出了一种可应用于体外组织模型的氧调节系统。我们制造了一种带有透气膜的微器件，使氧气能够扩散而无需与细胞直接接触。通过计算模拟和实验，我们验证了该器件内部形成了氧气浓度低于2% O₂的环境。将H9c2心脏成肌细胞置于该器件的缺氧环境中，并对其细胞活力进行了研究。我们预计，作为一种高效的氧调节系统，我们的系统将与一个研究缺氧诱导的人类生理和病理的平台相结合。