从芒果汁中分离的假肠膜明串珠菌 YB-2 中提取和表征右旋糖酐。
Extraction and characterization of dextran from Leuconostoc pseudomesenteroides YB-2 isolated from mango juice.
机构信息
Youjiang Medical University for Nationalities, Guangxi, Baise, 533000, PR China; Medical College of Guangxi University, Guangxi, Nanning, 530004, PR China.
Youjiang Medical University for Nationalities, Guangxi, Baise, 533000, PR China.
出版信息
Carbohydr Polym. 2019 Mar 1;207:218-223. doi: 10.1016/j.carbpol.2018.11.092. Epub 2018 Nov 29.
An exopolysaccharide (EPS)-producing strain of YB-2 isolated from mango juice was identified as Leuconostoc pseudomesenteroides. The molecular weight (Mw) of this EPS was 7.67×10 Da. Gas chromatography (GC) analysis confirmed the presence of only glucose monomers. Fourier transform infrared (FT-IR) spectroscopy and nuclear magnetic resonance (NMR) spectra displayed the glucan nature of the EPS with 96.8% α-(1→6) and 3.2% branching α-(1→3) linkages. Scanning electron microscopy (SEM) showed smooth surfaces and compact structure. The water solubility index (WSI) and water-holding capacity (WHC) of dextran were 97.48±2.46% and 287.51±7.93%, respectively. The rheological analysis of dextran elucidated a non-Newtonian pseudoplastic behavior. The dextran revealed an inhibitory activity against Escherichia coli and Staphylococcus aureus with minimal inhibitory concentrations (MIC) of 2.0 mg/mL and 3.0 mg/mL, respectively.
从芒果汁中分离到一株产胞外多糖（EPS）的 YB-2 菌株，被鉴定为肠膜明串珠菌。该 EPS 的分子量（Mw）为 7.67×10 Da。气相色谱（GC）分析证实只有葡萄糖单体存在。傅里叶变换红外（FT-IR）光谱和核磁共振（NMR）谱显示 EPS 具有葡聚糖性质，其中 96.8%的α-（1→6）和 3.2%的分支α-（1→3）键。扫描电子显微镜（SEM）显示出光滑的表面和紧凑的结构。葡聚糖的水溶性指数（WSI）和持水能力（WHC）分别为 97.48±2.46%和 287.51±7.93%。葡聚糖的流变分析表明其具有非牛顿假塑性行为。葡聚糖对大肠杆菌和金黄色葡萄球菌表现出抑制活性，最小抑菌浓度（MIC）分别为 2.0 mg/mL 和 3.0 mg/mL。
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