School of Sciences, P. P. Savani University, Surat 394125, Gujarat, India.
School of Agriculture, P. P. Savani University, Surat 394125, Gujarat, India.
Molecules. 2024 Feb 2;29(3):695. doi: 10.3390/molecules29030695.
The literature presents the preserving effect of biological coatings developed from various microbial sources. However, the presented work exhibits its uniqueness in the utilization of halophilic exopolysaccharides as food coating material. Moreover, such extremophilic exopolysaccharides are more stable and economical production is possible. Consequently, the aim of the presented research was to develop a coating material from marine exopolysaccharide (EPS). The significant EPS producers having antagonistic attributes against selected phytopathogens were screened from different marine water and soil samples. TSIS01 isolate revealed the maximum antagonism well and EPS production was selected further and characterized as MS01 by 16S rRNA analysis. EPS production was optimized and deproteinized EPS was assessed for biophysical properties. High performance thin layer chromatography (HPTLC) analysis revealed that EPS was a heteropolymer of glucose, galactose, mannose, and glucuronic acid. Fourier transform infrared spectroscopy, X-ray diffraction, and UV-visible spectra validated the presence of determined sugars. It showed high stability at a wide range of temperatures, pH and incubation time, ≈1.63 × 10 Da molecular weight, intermediate solubility index (48.2 ± 3.12%), low water holding capacity (12.4 ± 1.93%), and pseudoplastic rheologic shear-thinning comparable to xanthan gum. It revealed antimicrobial potential against human pathogens and antioxidants as well as anti-inflammatory potential. The biocontrol assay of EPS against phytopathogens revealed the highest activity against . The EPS-coated and control tomato fruits were treated with suspension to check the % disease incidence, which revealed a significant ( < 0.001) decline compared to uncoated controls. Moreover, it revealed shelf-life prolonging action on tomatoes comparable to xanthan gum and higher than chitosan. Consequently, the presented marine EPS was elucidated as a potent coating material to mitigate post-harvest losses.
该文献提出了从各种微生物来源开发的生物涂层的保存效果。然而，本工作利用嗜盐胞外多糖作为食品涂层材料，展示了其独特性。此外，这种极端嗜盐胞外多糖更稳定，并且可以进行更经济的生产。因此，本研究的目的是开发一种来自海洋胞外多糖（EPS）的涂层材料。从不同的海水和土壤样本中筛选出具有拮抗特性的显著 EPS 产生菌，以对抗选定的植物病原菌。TSIS01 分离株表现出最大的拮抗作用，进一步选择 EPS 产量并通过 16S rRNA 分析鉴定为 MS01。优化 EPS 生产并评估脱蛋白 EPS 的生物物理性质。高效薄层色谱（HPTLC）分析表明，EPS 是葡萄糖、半乳糖、甘露糖和葡萄糖醛酸的杂多糖。傅里叶变换红外光谱、X 射线衍射和紫外可见光谱验证了所确定糖的存在。它在较宽的温度、pH 和孵育时间范围内表现出高稳定性，分子量约为 1.63 × 10 Da，中等溶解度指数（48.2 ± 3.12%），低持水能力（12.4 ± 1.93%），并且类似黄原胶的假塑性剪切变稀流变学。它显示出对人类病原体和抗氧化剂以及抗炎剂的抗菌潜力。EPS 对植物病原菌的生物防治试验表明，对 的活性最高。用 悬浮液处理 EPS 涂层和对照番茄果实，以检查 %发病率，与未涂层对照相比，发病率显著（<0.001）下降。此外，它对番茄的货架期延长作用与黄原胶相当，高于壳聚糖。因此，所提出的海洋 EPS 被阐明为一种有效的涂层材料，可以减轻采后损失。
Zotero 插件