Zhou Bosen, Wang Changli, Yang Yi, Yu Wenna, Bin Xiaoyun, Song Gang, Du Renpeng
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Microbiology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China.
College of Basic Medical Sciences, Youjiang Medical University for Nationalities, Baise 533000, China.
Polymers (Basel). 2024 Aug 15;16(16):2314. doi: 10.3390/polym16162314.
An exopolysaccharide (EPS)-producing strain, identified as HDL-4, was isolated from litchi. After separation and purification, the structure and properties of HDL-4 EPS were characterized. The molecular weight of HDL-4 EPS was determined to be 1.9 × 10⁶ Da, with glucose as its monosaccharide component. Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance (NMR) analyses indicated that HDL-4 EPS was a D-glucan with α-(1→6) and α-(1→4) glycosidic bonds. X-ray diffraction (XRD) analysis revealed that HDL-4 EPS was amorphous. Scanning electron microscope (SEM) and atomic force microscope (AFM) observations showed that HDL-4 EPS possesses pores, irregular protrusions, and a smooth layered structure. Additionally, HDL-4 EPS demonstrated significant thermal stability, remaining stable below 288 °C. It exhibited a strong metal ion adsorption activity, emulsification activity, antioxidant activity, and water-retaining property. Therefore, HDL-4 EPS can be extensively utilized in the food and pharmaceutical industries as an additive and prebiotic.
从荔枝中分离出一株产胞外多糖（EPS）的菌株，鉴定为HDL-4。经过分离纯化后，对HDL-4 EPS的结构和性质进行了表征。HDL-4 EPS的分子量测定为1.9×10⁶ Da，其单糖组分为葡萄糖。傅里叶变换红外光谱（FT-IR）和核磁共振（NMR）分析表明，HDL-4 EPS是一种具有α-(1→6)和α-(1→4)糖苷键的D-葡聚糖。X射线衍射（XRD）分析表明HDL-4 EPS为无定形。扫描电子显微镜（SEM）和原子力显微镜（AFM）观察显示，HDL-4 EPS具有孔隙、不规则突起和平滑的层状结构。此外，HDL-4 EPS表现出显著的热稳定性，在288℃以下保持稳定。它具有很强的金属离子吸附活性、乳化活性、抗氧化活性和保水性能。因此，HDL-4 EPS作为添加剂和益生元可广泛应用于食品和制药行业。
