Environment Science and Engineering College, Yangzhou University, Yangzhou, Jiangsu 225009, China; State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, Jiangsu 210023, China; Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing 210095, China.
Environment Science and Engineering College, Yangzhou University, Yangzhou, Jiangsu 225009, China.
Ultrason Sonochem. 2019 May;53:68-76. doi: 10.1016/j.ultsonch.2018.12.034. Epub 2018 Dec 26.
For the first time, the inactivation of Microcystis aeruginosa using sono-Fenton process at low frequency high intensity (20 kHz, 0.42 W/mL) and high frequency low intensity (800 kHz, 0.07 W/mL) was investigated, respectively. 20 kHz sono-Fenton treatment successfully reduced cyanobacterial cell number from 4.19 × 10 cells/mL to 0.45 × 10 cells/mL within 5 min treatment. Alternatively, efficient performance of 800 kHz sono-Fenton process was observed to decrease Microcystis cell number to 2.33 × 10 cells/mL after 5 min inactivation, with lower energy cost. It was found that powerful 20 kHz sonication induced pore formation on the cell wall, leading to extracellular damage, while 800 kHz irradiation with low intensity triggered intracellular uptake of chemicals, suggesting endocytosis effects. Furthermore, sono-Fenton Processes were found to be affected by the concentrations of Fenton's reagent, and pre-sonication time. Although solo Fenton treatment released microcystins in water, the degradation of microcystin-LR were achieved using 20 and 800 kHz sono-Fenton processes, respectively. The results of this work showed that severe extracellular oxidation is the vital inactivation mechanism of 20 kHz sono-Fenton process, while the internal oxidation caused by intracellularly delivered Fenton reagents is suggested to be the main cause of 800 kHz sono-Fenton inactivation, leading to much lower energy cost. This work provides alternative methods to control harmful cyanobacteria in water towards effective treatment.
首次分别采用低频高强度（20 kHz，0.42 W/mL）和高频低强度（800 kHz，0.07 W/mL）的声芬顿工艺对铜绿微囊藻进行灭活研究。20 kHz 声芬顿处理可在 5 min 内将蓝藻细胞数从 4.19×10 cells/mL 成功减少至 0.45×10 cells/mL。相比之下，高效的 800 kHz 声芬顿工艺在 5 min 灭活后可将微囊藻细胞数减少至 2.33×10 cells/mL，能耗更低。研究发现，强大的 20 kHz 超声在细胞壁上诱导形成孔，导致细胞外损伤，而低强度的 800 kHz 辐照则引发化学物质的细胞内摄取，提示存在胞吞作用。此外，声芬顿工艺受芬顿试剂浓度和预超声时间的影响。虽然单独的芬顿处理会使水中释放微囊藻毒素，但 20 和 800 kHz 声芬顿工艺分别可实现微囊藻毒素-LR 的降解。本研究结果表明，剧烈的细胞外氧化是 20 kHz 声芬顿工艺的重要灭活机制，而由细胞内传递的芬顿试剂引起的细胞内氧化则是 800 kHz 声芬顿灭活的主要原因，这导致了更低的能耗。本工作为控制水中有害蓝藻提供了替代方法，以期实现有效处理。
