Asgari Rezvan, Mansouri Kamran, Bakhtiari Mitra, Vaisi-Raygani Asad
Medical Biology Research Center (MBRC), Kermanshah University of Medical Sciences, Kermanshah, Iran.
Department of Anatomical Sciences & Cell Biology, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Mol Biol Rep. 2019 Feb;46(1):1099-1105. doi: 10.1007/s11033-018-4568-y. Epub 2019 Jan 2.
CD147 plays an important role in germ cells migration and survival/apoptosis during the spermatogenesis process. However, to best of our knowledge, there is no report on the exact role of CD147 gene in the regulation of germ cells apoptosis through matrix metalloproteinases (MMPs). So, the current study aims to evaluate the role of CD147 gene expression in the regulation of germ cells apoptosis in conjunction with MMPs. Real-Time PCR was applied to investigate the expression of CD147, MMP2, MMP7, and MMP9 genes in the azoospermic patients and fertile males. Receiver-operating characteristic curve was used to interpret gene expression data. According to our results, a significant decrease in the expression of CD147 gene and an increase in MMPs genes expression were observed in infertile patients compared to fertile males. These results proved this fact that the CD147 gene has an important role in the regulation of germ cells apoptosis via a MMPs-dependent pathway.
CD147在精子发生过程中的生殖细胞迁移和存活/凋亡中发挥着重要作用。然而，据我们所知，尚无关于CD147基因通过基质金属蛋白酶（MMPs）调控生殖细胞凋亡的确切作用的报道。因此，本研究旨在评估CD147基因表达在联合MMPs调控生殖细胞凋亡中的作用。应用实时荧光定量PCR检测无精子症患者和正常生育男性中CD147、MMP2、MMP7和MMP9基因的表达。采用受试者工作特征曲线解释基因表达数据。根据我们的结果，与正常生育男性相比，不育患者中CD147基因表达显著降低，MMPs基因表达增加。这些结果证明了CD147基因通过MMPs依赖途径在调控生殖细胞凋亡中具有重要作用。
