Bezerra Luana G P, Souza Ana L P, Lago Arthur E A, Campos Livia B, Nunes Talita L, Paula Valéria V, Oliveira Moacir F, Silva Alexandre R
Laboratory of Animal Germplasm Conservation-LCGA, Department of Animal Sciences, Centro de Ciências Agrárias, Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, Rio Grande do Norte, Brazil.
Biopreserv Biobank. 2019 Apr;17(2):143-147. doi: 10.1089/bio.2018.0096. Epub 2019 Jan 2.
The effect of Equex STM paste supplementation on the Tris-extender for collared peccaries' semen cryopreservation was assessed. Semen from 12 mature individuals was obtained by electroejaculation and evaluated for morphology, membrane integrity, osmotic response, and sperm kinetic metrics. Samples were diluted in Tris plus 20% egg yolk and divided into three aliquots. The first aliquot was without any supplementation, the second and third contained 0.5 and 1.0% Equex STM, respectively. The samples were added with 3% glycerol, frozen in liquid nitrogen, thawed, and assessed for the same parameters after a thermal resistance test (TRT) for 120 minutes. Similar values were detected for the different treatments immediately after thawing, except for the amplitude lateral head that was reduced in samples containing Equex (p < 0.05). During TRT, samples containing Equex were more efficient in preserving the sperm motility (at 0.5%: 25.5% ± 4.4%; at 1%: 33.3% ± 6.3%) at 30 minutes, in comparison with the control group (16.6% ± 6.0%), in which sperm motility decreased at 15 minutes (p < 0.05). Moreover, Equex, especially at 0.5% concentration, was able to maintain plasma membrane integrity and sperm motility in all the samples after incubation for 60 minutes. In conclusion, we recommend the addition of Equex STM at 0.5% to the Tris-extender to improve post-thawing sperm longevity in collared peccaries.
评估了在白唇野猪精液冷冻保存的Tris稀释液中添加Equex STM糊剂的效果。通过电刺激采精获取12只成年白唇野猪的精液，并对其形态、膜完整性、渗透反应和精子运动指标进行评估。样本在含20%蛋黄的Tris中稀释，并分为三等份。第一份不添加任何物质，第二份和第三份分别含有0.5%和1.0%的Equex STM。样本添加3%甘油后，在液氮中冷冻、解冻，并在进行120分钟耐热性测试（TRT）后对相同参数进行评估。解冻后立即检测到不同处理的相似值，但含Equex的样本中精子头部侧摆幅度降低（p < 0.05）。在TRT过程中，与对照组（16.6%±6.0%）相比，含Equex的样本在30分钟时更有效地保持了精子活力（0.5%时：25.5%±4.4%；1%时：33.3%±6.3%），对照组在15分钟时精子活力下降（p < 0.
