肿瘤诱导血小板中下调的 lncRNA ROR 作为鼻咽癌的液体活检生物标志物。
Down-regulated lncRNA ROR in tumor-educated platelets as a liquid-biopsy biomarker for nasopharyngeal carcinoma.
机构信息
Department of Laboratory Medicine, Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Department of Laboratory Medicine, Wuhan Jiangxia Hospital of Traditional Chinese Medicine, Wuhan, 430022, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 2023 Jul;149(8):4403-4409. doi: 10.1007/s00432-022-04350-1. Epub 2022 Sep 15.
PURPOSES
To evaluate the diagnostic value of tumor-educated platelets (TEP) lncRNA ROR for nasopharyngeal carcinoma (NPC).
METHODS
Quantitative real-time PCR was used to determine the expression level of TEP lncRNA ROR in NPC patients (n = 50) as compared to normal subjects (n = 33). The ROC curve analysis was performed to assess the diagnostic value of TEP lncRNA ROR for NPC. Correlations between TEP lncRNA ROR and clinical parameters were further analyzed.
RESULTS
The median of TEP lncRNA ROR was significantly lower in NPC patients than that in normal subjects (0.0209 vs 0.0610, p = 0.0019), while no significant difference was found in plasma lncRNA ROR. ROC analysis showed that TEP lncRNA ROR had a sensitivity of 60%, specificity of 70%, and accuracy of 63.9% in diagnosing NPC, and the area under ROC curve (AUC) was 0.70. The expression level of TEP lncRNA ROR in NPC showed no significant difference among different TNM stages. However, low level of TEP lncRNA ROR correlated well with positive Epstein-Barr virus (EBV) DNA (kappa value = 0.314, p = 0.06), TEP lncRNA ROR and EBV DNA had similar diagnostic positive rate (58.3%) for NPC, and the combination of TEP lncRNA ROR and EBV DNA increased the positive rate to 74%.
CONCLUSION
The expression level of TEP lncRNA ROR was down-regulated in NPC and the diagnostic value of TEP lncRNA ROR was similar to EBV DNA. Our study indicated that TEP lncRNA ROR might serve as a novel type of liquid biopsy biomarker in diagnosis of NPC patients.
目的
评估肿瘤衍生血小板（TEP）lncRNA ROR 对鼻咽癌（NPC）的诊断价值。
方法
采用实时定量 PCR 检测 50 例 NPC 患者和 33 例正常对照的 TEP lncRNA ROR 表达水平。绘制 ROC 曲线评估 TEP lncRNA ROR 对 NPC 的诊断价值，并进一步分析 TEP lncRNA ROR 与临床参数的相关性。
结果
与正常对照组相比，NPC 患者 TEP lncRNA ROR 的中位数明显降低（0.0209 比 0.0610，p=0.0019），而血浆 lncRNA ROR 无显著差异。ROC 分析显示，TEP lncRNA ROR 诊断 NPC 的敏感性为 60%，特异性为 70%，准确性为 63.9%，ROC 曲线下面积（AUC）为 0.70。不同 TNM 分期 NPC 患者的 TEP lncRNA ROR 表达水平无显著差异。然而，低水平的 TEP lncRNA ROR 与 EBV DNA 阳性（kappa 值=0.314，p=0.06）密切相关，TEP lncRNA ROR 和 EBV DNA 对 NPC 的诊断阳性率相似（58.3%），联合检测 TEP lncRNA ROR 和 EBV DNA 可将阳性率提高至 74%。
结论
TEP lncRNA ROR 在 NPC 中表达下调，其诊断价值与 EBV DNA 相似。本研究表明，TEP lncRNA ROR 可能成为 NPC 患者新型液体活检标志物。
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