Kanaani J, Ginsburg H
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
Biochem Int. 1988 Sep;17(3):451-9.
The ATP concentration of malaria-infected erythrocytes changes substantially with parasite development. These alterations have been attributed to a decline in host cell [ATP], but have not been tested critically hitherto. A method for the compartmental analysis of ATP in malaria (Plasmodium falciparum)-infected human red blood cells has been developed using Sendai virus to permeabilize the host erythrocyte membrane. Permeabilization and release of host cytosol was complete within 6 to 8 minutes and ATP was measured by the luciferin-luciferase bioluminescence assay in the lysate and in the pellet. Equal ATP concentrations were found in host and parasite compartments at the trophozoite and schizont stages. Both were lower than those detected in uninfected cells. Other methods for compartment analysis of ATP are presented and discussed.
疟原虫感染的红细胞中的三磷酸腺苷（ATP）浓度会随着寄生虫的发育而发生显著变化。这些变化被归因于宿主细胞内ATP含量的下降，但迄今为止尚未得到严格验证。利用仙台病毒使宿主红细胞膜通透化，已开发出一种用于对感染疟疾（恶性疟原虫）的人类红细胞中的ATP进行区室分析的方法。在6至8分钟内宿主细胞质完全通透并释放出来，通过荧光素 - 荧光素酶生物发光测定法对裂解物和沉淀中的ATP进行测量。在滋养体和裂殖体阶段，宿主和寄生虫区室中的ATP浓度相等。两者均低于未感染细胞中检测到的浓度。还介绍并讨论了其他用于ATP区室分析的方法。
