NanoBioCel Group, Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy , University of the Basque Country UPV/EHU , Vitoria-Gasteiz 01006 , Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials, and Nanomedicine, CIBER-BBN , Vitoria-Gasteiz 01006 , Spain.
Mol Pharm. 2019 Feb 4;16(2):834-845. doi: 10.1021/acs.molpharmaceut.8b01126. Epub 2019 Jan 10.
Islet transplantation has shown to be a successful alternative in type 1 diabetes treatment, but donor scarcity precludes its worldwide clinical translation. Stem cells are an unlimited source that could circumvent the lack of donors if complete differentiation into insulin-producing cells (IPCs) could be accomplished. We have performed the differentiation of mesenchymal stem cells (MSCs) from different sources into IPCs within three-dimensional (3D) alginate matrixes. We quantified an increased insulin release at the final stage of differentiation compared to undifferentiated MSCs, which is more pronounced in IPCs differentiated from pancreatic-derived MSCs tissues. Moreover, the addition of hyaluronic acid (HA) in alginate microcapsules enhanced, even more, the insulin release from the final IPCs, independent of the MSC source. We can conclude that MSCs can be differentiated into IPCs within alginate microcapsules, enhancing insulin release when HA is present in the 3D alginate matrixes.
胰岛移植已被证明是治疗 1 型糖尿病的一种成功方法，但由于供体稀缺，限制了其在全球范围内的临床应用。干细胞是一种无限供应的资源，如果能够将其完全分化为胰岛素生成细胞（IPCs），就可以解决供体不足的问题。我们已经成功地将来自不同来源的间充质干细胞（MSCs）在三维（3D）藻酸盐基质中分化为 IPCs。我们发现，与未分化的 MSCs 相比，在分化的最后阶段胰岛素的释放量增加，而来源于胰腺来源的 MSCs 组织的 IPCs 中这种增加更为明显。此外，在藻酸盐微胶囊中添加透明质酸（HA），即使在 3D 藻酸盐基质中存在 HA 时，也能进一步增强最终 IPC 中的胰岛素释放。我们可以得出结论，MSCs 可以在藻酸盐微胶囊中分化为 IPCs，当 HA 存在于 3D 藻酸盐基质中时，会增强胰岛素的释放。
