Park Junha, Choe Goeun, Oh Seulgi, Lee Jae Young
School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea.
ACS Biomater Sci Eng. 2020 Dec 14;6(12):6938-6948. doi: 10.1021/acsbiomaterials.0c01489. Epub 2020 Dec 2.
Mesenchymal stem-cell (MSC)-based therapies have been recognized as promising strategies for the treatment of various injuries or diseases because of their unique characteristics, such as self-renewal, differentiation potential, and secretion of various bioactive molecules. However, MSC transplantation often results in low efficacy, including a cell viability loss and a low therapeutic activity. Alternatively, MSC spheroids have been studied to improve the viability and therapeutic activity of MSCs. Also, microencapsulation of cells can protect and retain the cells from harsh environments after transplantation. Here, MSC spheroids were formed in hyaluronic acid/alginate (HA@Alg) core-shell microcapsules and employed for neovascularization. A well-defined core-shell structure of HA@Alg microcapsules was produced by optimizing various electrospraying conditions. MSC spheroids could be spontaneously formed in the HA core of the microcapsules after 1 day of incubation. Enhanced secretion of various growth factors was found from MSC spheroids in HA@Alg. plug assay revealed the significant promotion of angiogenesis by MSC spheroids in HA@Alg compared to that by the controls (, MSCs and MSC spheroids), which is likely because of the better retention of MSC spheroid forms in the microcapsules. Thus, the HA@Alg microcapsules embedding MSC spheroids will be greatly beneficial for various stem cell-based therapies.
基于间充质干细胞（MSC）的疗法因其独特特性，如自我更新、分化潜能以及分泌多种生物活性分子，已被视为治疗各种损伤或疾病的有前景的策略。然而，MSC移植往往疗效不佳，包括细胞活力丧失和治疗活性较低。另外，人们已对MSC球状体进行研究以提高MSC的活力和治疗活性。而且，细胞微囊化可在移植后保护细胞并使其免受恶劣环境影响。在此，MSC球状体在透明质酸/藻酸盐（HA@Alg）核壳微胶囊中形成并用于血管新生。通过优化各种电喷雾条件制备出结构明确的HA@Alg微胶囊核壳结构。孵育1天后，MSC球状体可在微胶囊的HA核中自发形成。在HA@Alg中发现MSC球状体分泌的各种生长因子有所增加。栓塞试验显示，与对照组（即MSC和MSC球状体）相比，HA@Alg中的MSC球状体对血管生成有显著促进作用，这可能是因为微胶囊中MSC球状体形态保留得更好。因此，包埋MSC球状体的HA@Alg微胶囊对各种基于干细胞的疗法将大有裨益。
