Department of Bacteriology, University of Wisconsin-Madison, 1550 Linden Drive, Madison, WI 53706, USA.
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53706, USA.
FEMS Microbiol Lett. 2019 Feb 1;366(4). doi: 10.1093/femsle/fny296.
Salmonellosis outbreaks associated with sprouted legumes have been a food safety concern for over two decades. Despite evidence that Salmonella enterica triggers biotic plant defense pathways, it has remained unclear how plant defenses impact Salmonella growth on sprouted legumes. We used Medicago truncatula mutants in which the gene for the flagellin receptor FLS2 was disrupted to demonstrate that plant defenses triggered by FLS2 elicitation do not impact the growth of Salmonella enterica serovar Typhimurium ATCC 14028S. As a control, we tested the growth of Salmonella enterica serovar Typhimurium LT2, which has a defect in rpoS that increases its sensitivity to reactive oxygen species. LT2 displayed enhanced growth on M. truncatula FLS2 mutants in comparison to wild-type M. truncatula. We hypothesize that these growth differences are primarily due to differences in 14028S and LT2 reactive oxygen species sensitivity. Results from this study show that FLS2-mediated plant defenses are ineffective in inhibiting growth of Salmonella entrica 14028S.
与豆芽有关的沙门氏菌病爆发已经成为了二十多年来的食品安全关注点。尽管有证据表明沙门氏菌会触发生物植物防御途径，但植物防御如何影响豆芽上沙门氏菌的生长仍不清楚。我们使用了拟南芥的突变体，其中 flagellin 受体 FLS2 的基因被破坏，以证明由 FLS2 引发的植物防御不会影响肠炎沙门氏菌血清型 Typhimurium ATCC 14028S 的生长。作为对照，我们测试了肠炎沙门氏菌血清型 LT2 的生长情况，LT2 的 rpoS 缺陷使其对活性氧更敏感。与野生型拟南芥相比，LT2 在拟南芥 FLS2 突变体上的生长增强。我们假设这些生长差异主要是由于 14028S 和 LT2 对活性氧的敏感性不同。这项研究的结果表明，FLS2 介导的植物防御在抑制肠炎沙门氏菌 14028S 的生长方面无效。
