通过稀有限制性酶切位点扩增进行DNA指纹分析。
DNA fingerprinting by infrequent-restriction-site amplification.
作者信息
Mazurek G H, Reddy V, Marston B J, Haas W H, Crawford J T
机构信息
National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1996 Oct;34(10):2386-90. doi: 10.1128/jcm.34.10.2386-2390.1996.
Abstract
Identification of bacterial strains by DNA fingerprinting facilitates epidemiologic studies and improves disease control. For some species of organisms, no typing method is available; for others, typing methods are tedious. We developed a method of amplifying DNA sequences flanking infrequent restriction sites by PCR and used the method to produce strain-specific electrophoretic patterns from crude bacterial lysates. This method of fingerprinting is rapid, sensitive, and widely applicable. Identical enzymes, adaptors, primers, and PCR conditions were used to characterize 32 Mycobacterium avium-M. intracellulare isolates, 4 Pseudomonas aeruginosa isolates, and 4 Staphylococcus aureus isolates.
摘要
通过DNA指纹图谱鉴定细菌菌株有助于流行病学研究并改善疾病控制。对于某些生物体物种，没有可用的分型方法；对于其他物种，分型方法很繁琐。我们开发了一种通过PCR扩增罕见限制位点侧翼DNA序列的方法，并使用该方法从粗制细菌裂解物中产生菌株特异性电泳图谱。这种指纹图谱方法快速、灵敏且广泛适用。使用相同的酶、衔接子、引物和PCR条件对32株鸟分枝杆菌-胞内分枝杆菌分离株、4株铜绿假单胞菌分离株和4株金黄色葡萄球菌分离株进行了鉴定。
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