Miao Zongning, Sun Hongli, Xue Yifeng
1The Stem Cell Research Laboratory, Wuxi Third People's Hospital, Wuxi, China.
2Department of Clinical Laboratory, Affiliated Wuxi People's Hospital, Nanjing Medical University, Qingyang Road 299#, Wuxi, Jiangsu Province China.
Tissue Eng Regen Med. 2017 Mar 2;14(2):143-151. doi: 10.1007/s13770-017-0025-6. eCollection 2017 Apr.
Mesenchymal stem cells (MSCs) can be obtained from a variety of human tissues. Placenta has become an attractive stem cell source for potential applications in regenerative medicine and tissue engineering. The aim of this study was to localize and characterize MSCs within human chorionic membranes (hCMSCs). For this purpose, immunofluorescence labeling with CD105 and CD90 were used to determine the distribution of MSCs in chorionic membranes tissue. A medium supplemented with a synthetic serum and various concentrations of neurotrophic factors and cytokines was used to induce hCMSCs to neural cells. The results showed that the CD90 positive cells were scattered in the chorionic membranes tissue, and the CD105 positive cells were mostly located around the small blood vessels. hCMSCs expressed typical mesenchymal markers (CD73, CD90, CD105, CD44 and CD166) but not hematopoietic markers (CD45, CD34) and HLA-DR. hCMSCs differentiated into adipocytes, osteocytes, chondrocytes, and neuronal cells, as revealed by morphological changes, cell staining, immunofluorescence analyses, and RT-PCR showing the tissue-specific gene presence for differentiated cell lineages after the treatment with induce medium. Human chorionic membranes may be the source of MSCs for treatment of nervous system injury.
间充质干细胞（MSCs）可从多种人体组织中获取。胎盘已成为再生医学和组织工程潜在应用中一种有吸引力的干细胞来源。本研究的目的是在人绒毛膜（hCMSCs）中定位和鉴定间充质干细胞。为此，使用CD105和CD90免疫荧光标记来确定间充质干细胞在绒毛膜组织中的分布。使用添加了合成血清以及不同浓度神经营养因子和细胞因子的培养基诱导hCMSCs分化为神经细胞。结果显示，CD90阳性细胞散在于绒毛膜组织中，而CD105阳性细胞大多位于小血管周围。hCMSCs表达典型的间充质标志物（CD73、CD90、CD105、CD44和CD166），但不表达造血标志物（CD45、CD34）和HLA - DR。形态学变化、细胞染色、免疫荧光分析以及RT - PCR显示，在用诱导培养基处理后，hCMSCs分化为脂肪细胞、骨细胞、软骨细胞和神经元细胞，且分化细胞谱系存在组织特异性基因，这表明hCMSCs可分化为这些细胞。人绒毛膜可能是用于治疗神经系统损伤的间充质干细胞来源。
