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人羊膜（AM）、脐带（UC）、绒毛膜（CM）和蜕膜（DC）来源的间充质干细胞成骨分化能力的比较。

Comparison of osteogenic differentiation capacity in mesenchymal stem cells derived from human amniotic membrane (AM), umbilical cord (UC), chorionic membrane (CM), and decidua (DC).

作者信息

Shen Chongyang, Yang Chuan, Xu Shijun, Zhao Hai

机构信息

1Basic Medicine School, Chengdu University of Traditional Chinese Medicine, Chengdu, People's Republic of China.

2Key Laboratory of Obstetric and Gynecologic and Pediatric Diseases and Birth Defects of the Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, People's Republic of China.

出版信息

Cell Biosci. 2019 Feb 11;9:17. doi: 10.1186/s13578-019-0281-3. eCollection 2019.

DOI:10.1186/s13578-019-0281-3
PMID:30792848
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6371545/
Abstract

BACKGROUND

Mesenchymal stem cells (MSCs) have been extensively explored as a promising therapeutic agent in the field of bone tissue engineering due to their osteogenic differentiation ability. In this study, the osteogenic differential ability and the effect of fibronectin and laminin on the osteogenic differentiation in four types of MSCs derived from placental tissue are compared to determine the ideal source for bone reconstruction tissue engineering.

RESULTS

The present study examines the osteogenic differentiation levels of four types of MSCs using alizarin red staining and quantifies the calcium levels and alkaline phosphatase (ALP) activity. In addition, this study examines the osteoblast differentiation protein markers osterix, collagen I, osteopontin, and osteocalcin using a Western blot assay. qPCR and EdU labeling assays were employed to identify the kinetics of osteogenic differentiation. Calcium deposit levels, ALP activity, and osteopontin and osteocalcin concentrations were determined to confirm the role of Extracellular matrix (ECM) components role on the osteogenic differentiation of MSCs. The data demonstrated that MSCs isolated from different layers of placenta had different potentials to differentiate into osteogenic cells. Importantly, AM-MSCs and UC-MSCs differentiated into the osteoblast stage more efficiently and quickly than CM-MSCs and DC-MSCs, which was associated with a decrease in their proliferation ability. Among the different types of MSCs, AM-MSCs and UC-MSCs had a higher osteogenic differentiation potential induced by fibronectin due to enhanced phosphorylation during the Akt and ERK pathways.

CONCLUSIONS

Taken together, these results indicate that AM-MSCs and UC-MSCs possess a higher osteogenic potential, and fibronectin can robustly enhance the osteogenic potential of the Akt and ERK pathways.

摘要

背景

间充质干细胞（MSCs）因其成骨分化能力，在骨组织工程领域作为一种有前景的治疗剂受到广泛探索。在本研究中，比较了纤连蛋白和层粘连蛋白对四种源自胎盘组织的间充质干细胞成骨分化的影响及其成骨分化能力，以确定骨重建组织工程的理想来源。

结果

本研究使用茜素红染色检测了四种间充质干细胞的成骨分化水平，并对钙水平和碱性磷酸酶（ALP）活性进行了定量。此外，本研究使用蛋白质免疫印迹法检测了成骨细胞分化蛋白标志物osterix、I型胶原蛋白、骨桥蛋白和骨钙素。采用qPCR和EdU标记试验来确定成骨分化的动力学。测定钙沉积水平、ALP活性以及骨桥蛋白和骨钙素浓度，以确认细胞外基质（ECM）成分在间充质干细胞成骨分化中的作用。数据表明，从胎盘不同层分离的间充质干细胞向成骨细胞分化的潜能不同。重要的是，羊膜间充质干细胞（AM-MSCs）和脐带来源间充质干细胞（UC-MSCs）比绒毛膜间充质干细胞（CM-MSCs）和蜕膜间充质干细胞（DC-MSCs）更有效、更快地分化为成骨细胞阶段，这与它们增殖能力的降低有关。在不同类型的间充质干细胞中，由于Akt和ERK途径中磷酸化增强，AM-MSCs和UC-MSCs在纤连蛋白诱导下具有更高的成骨分化潜能。

结论

综上所述，这些结果表明，AM-MSCs和UC-MSCs具有更高的成骨潜能，并且纤连蛋白可以显著增强Akt和ERK途径的成骨潜能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/f373ab18f482/13578_2019_281_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/7336f626197b/13578_2019_281_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/f786113e3852/13578_2019_281_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/ef40653af5d1/13578_2019_281_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/bb7399e3fbb7/13578_2019_281_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/6eb8039a1d80/13578_2019_281_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/e21a225e99f6/13578_2019_281_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/7c9453222220/13578_2019_281_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/f373ab18f482/13578_2019_281_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/7336f626197b/13578_2019_281_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/f786113e3852/13578_2019_281_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/ef40653af5d1/13578_2019_281_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/bb7399e3fbb7/13578_2019_281_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/6eb8039a1d80/13578_2019_281_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/e21a225e99f6/13578_2019_281_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/7c9453222220/13578_2019_281_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dd4c/6371545/f373ab18f482/13578_2019_281_Fig8_HTML.jpg

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