Poloni A, Rosini V, Mondini E, Maurizi G, Mancini S, Discepoli G, Biasio S, Battaglini G, Berardinelli E, Serrani F, Leoni P
Clinica di Ematologia, Dipartimento Scienze Mediche e Chirurgiche, Universita Politecnica delle Marche, Ancona, Italy.
Cytotherapy. 2008;10(7):690-7. doi: 10.1080/14653240802419310.
Mesenchymal stromal cells (MSC) have been identified in a variety of fetal and adult tissues, including bone marrow (BM), fetal blood and liver. We report on the isolation, expansion and differentiation in vitro of MSC-like cells from chorionic villi (CV).
We evaluated 10 samples of CV collected at the first trimester (gestational age 11-13 weeks). We only used cells taken from back-up culture after a successful karyotype analysis. CV cells were characterized by morphologic, immunophenotypic and molecular analysis. The differentiation ability of mesenchymal and neural lineages was detected using specific culture conditions. Cell expansion was assessed after plating cells at different densities in different media, supplemented with animal and human serum.
CV cells showed a homogeneous population of spindle-shaped cells after the first passage. Cells expressed CD90, CD105, CD73, CD44, CD29 and CD13 but not CD45, CD14, CD34 and CD117. They expressed Oct-4, Rex-1, GATA-4 and nestin, which characterize the undifferentiated stem cell state. They differentiated into osteocytes, adipocytes, chondrocytes and neuronal cells. Cell expansion was greater than that of adult BM-derived MSC, 9 logs with fetal bovine serum and 6 logs with human serum. Despite their high proliferative capacity, we did not observe any karyotypic abnormalities after culture.
Our study shows that CV cells have better potential for expansion than adult stem cells. They can proliferate in a medium with human allogeneic serum and can differentiate into mesenchymal and neural lineages. CV cells may be an excellent cell source for therapeutic applications.
间充质基质细胞（MSC）已在多种胎儿和成人组织中被鉴定出来，包括骨髓（BM）、胎儿血液和肝脏。我们报告了从绒毛膜绒毛（CV）中分离、扩增和体外分化出类似MSC的细胞。
我们评估了孕早期（妊娠11 - 13周）收集的10份CV样本。我们仅使用在成功进行核型分析后从备用培养物中获取的细胞。通过形态学、免疫表型和分子分析对CV细胞进行表征。使用特定培养条件检测间充质和神经谱系的分化能力。在不同密度接种细胞于添加动物和人血清的不同培养基中后评估细胞扩增情况。
首次传代后，CV细胞呈现出均一的纺锤形细胞群体。细胞表达CD90、CD105、CD73、CD44、CD29和CD13，但不表达CD45、CD14、CD34和CD117。它们表达Oct - 4、Rex - 1、GATA - 4和巢蛋白，这些是未分化干细胞状态的特征。它们可分化为骨细胞、脂肪细胞、软骨细胞和神经细胞。细胞扩增能力强于成人BM来源的MSC，使用胎牛血清时扩增9个对数级，使用人血清时扩增6个对数级。尽管其增殖能力高，但培养后我们未观察到任何核型异常。
我们的研究表明，CV细胞比成体干细胞具有更好的扩增潜力。它们可在含人同种异体血清的培养基中增殖，并可分化为间充质和神经谱系。CV细胞可能是治疗应用的优良细胞来源。
